Neurite guidance by the FnC repeat of human tenascin-C: Neurite attraction vs. neurite retention

Hsing Yin Liu, Alam Nur-E-Kamal, Melitta Schachner, Sally Meiners

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The alternatively spliced fibronectin type-III repeat C of human tenascin-C (fnC) provides directional cues to elongating neurites in vitro. When given a choice at an interface with poly L-lysine (PLL), rat cerebellar granule neurites preferentially crossed onto fnC (defined herein as neurite attraction) whereas neurites originating on fnC preferentially remained on fnC (defined as neurite retention). Guidance motifs were further refined using synthetic peptides spanning the sequence of fnC. We found that a peptide with amino acid sequence DINPYGFTVSWMASE was sufficient to attract and retain neurites. Peptides with alterations in NPYG facilitated neurite retention but not attraction and, conversely, molecules with alterations in ASE facilitated neurite attraction but not retention. Hence neurite attraction and neurite retention mediated by fnC are separable events that can be independently regulated. This property may prove valuable for the strategic design of peptide reagents for use in strategies to facilitate directed axonal regrowth following CNS injury.

Original languageEnglish (US)
Pages (from-to)1863-1872
Number of pages10
JournalEuropean Journal of Neuroscience
Issue number8
StatePublished - Oct 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


  • Extracellular matrix
  • FN-III repeat
  • Peptide
  • Rat


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