Neurovascular Relationships in AGEs-Based Models of Proliferative Diabetic Retinopathy

Juan S. Peña, Ranjini K. Ramanujam, Rebecca A. Risman, Valerie Tutwiler, Francois Berthiaume, Maribel Vazquez

Research output: Contribution to journalArticlepeer-review

Abstract

Diabetic retinopathy affects more than 100 million people worldwide and is projected to increase by 50% within 20 years. Increased blood glucose leads to the formation of advanced glycation end products (AGEs), which cause cellular and molecular dysfunction across neurovascular systems. These molecules initiate the slow breakdown of the retinal vasculature and the inner blood retinal barrier (iBRB), resulting in ischemia and abnormal angiogenesis. This project examined the impact of AGEs in altering the morphology of healthy cells that comprise the iBRB, as well as the effects of AGEs on thrombi formation, in vitro. Our results illustrate that AGEs significantly alter cellular areas and increase the formation of blood clots via elevated levels of tissue factor. Likewise, AGEs upregulate the expression of cell receptors (RAGE) on both endothelial and glial cells, a hallmark biomarker of inflammation in diabetic cells. Examining the effects of AGEs stimulation on cellular functions that work to diminish iBRB integrity will greatly help to advance therapies that target vision loss in adults.

Original languageEnglish (US)
Article number63
JournalBioengineering
Volume11
Issue number1
DOIs
StatePublished - Jan 2024
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering

Keywords

  • RAGE
  • advanced glycation end products
  • diabetic retinopathy
  • inner blood retinal barrier
  • vascular disease

Fingerprint

Dive into the research topics of 'Neurovascular Relationships in AGEs-Based Models of Proliferative Diabetic Retinopathy'. Together they form a unique fingerprint.

Cite this