TY - JOUR
T1 - New core promoter element in RNA polymerase II-dependent transcription
T2 - Sequence-specific DNA binding by transcription factor IIB
AU - Lagrange, Thierry
AU - Kapanidis, Achillefs N.
AU - Tang, Hong
AU - Reinberg, Danny
AU - Ebright, Richard H.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves α-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.
AB - A sequence element located immediately upstream of the TATA element, and having the consensus sequence 5'-G/C-G/C-G/A-C-G-C-C-3', affects the ability of transcription factor IIB to enter transcription complexes and support transcription initiation. The sequence element is recognized directly by the transcription factor IIB. Recognition involves α-helices 4' and 5' of IIB, which comprise a helix-turn-helix DNA-binding motif. These observations establish that transcription initiation involves a fourth core promoter element, the IIB recognition element (BRE), in addition to the TATA element, the initiator element, and the downstream promoter element, and involves a second sequence-specific general transcription factor, IIB, in addition to transcription factor IID.
KW - DNA binding
KW - Helix- turn-helix
KW - IIB recognition element
KW - TATA element
KW - Transcription factor IIB
UR - http://www.scopus.com/inward/record.url?scp=0031882652&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031882652&partnerID=8YFLogxK
U2 - 10.1101/gad.12.1.34
DO - 10.1101/gad.12.1.34
M3 - Article
C2 - 9420329
AN - SCOPUS:0031882652
SN - 0890-9369
VL - 12
SP - 34
EP - 44
JO - Genes and Development
JF - Genes and Development
IS - 1
ER -