New transmembrane AMPA receptor regulatory protein isoform, γ-7, differentially regulates AMPA receptors

Akihiko S. Kato, Wei Zhou, Aaron D. Milstein, Mike D. Knierman, Edward R. Siuda, Joe E. Dotzlaf, Hong Yu, John E. Hale, Eric S. Nisenbaum, Roger A. Nicoll, David S. Bredt

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


AMPA-type glutamate receptors (GluRs) mediate most excitatory signaling in the brain and are composed of GluR principal subunits and transmembrane AMPA receptor regulatory protein (TARP) auxiliary subunits. Previous studies identified four mammalian TARPs, γ-2 (or stargazin), γ-3, γ-4, and γ-8, that control AMPA receptor trafficking, gating, and pharmacology. Here, we explore roles for the homologous γ-5 and γ-7 proteins, which were previously suggested not to serve as TARPs. Western blotting reveals high levels of γ-5 and γ-7 in the cerebellum, where γ-7 is enriched in Purkinje neurons in the molecular layer and glomerular synapses in the granule cell layer. Immunoprecipitation proteomics shows that cerebellar γ-7 avidly and selectively binds to AMPA receptor GluR subunits and also binds to the AMPA receptor clustering protein, postsynaptic density-95 (PSD-95). Furthermore, γ-7 occurs together with PSD-95 and AMPA receptor subunits in purified postsynaptic densities. In heterologous cells, γ-7 but not γ-5 greatly enhances AMPA receptor glutamate-evoked currents and modulates channel gating. In granule cells from stargazer mice, transfection of γ-7 but not γ-5 increases AMPA receptor-mediated currents. Compared with stargazin, γ-7 differentially modulates AMPA receptor glutamate affinity and kainate efficacy. These studies define γ-7 as a new member of the TARP family that can differentially influence AMPA receptors in cerebellar neurons.

Original languageEnglish (US)
Pages (from-to)4969-4977
Number of pages9
JournalJournal of Neuroscience
Issue number18
StatePublished - May 2 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


  • Auxiliary subunit
  • Cerebellum
  • Excitatory amino acid
  • Glutamate receptor
  • Ion channel
  • Mass spectroscopy


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