Abstract
Callus of Nicotiana tabacum SRI, a mutant with maternally inherited streptomycin resistance, was induced from leaf sections. Callus pieces were mutagenised with N-ethyl-N-nitrosourea and inoculated onto a shoot-induction medium on which calli are normally green. White callus sectors were observed in the mutagenised cultures, and white and variegated shoots were regenerated from these sectored calli. The SR1-A10 line regenerated a chimeric shoot with white leaf margins. The chimeric shoot was grafted onto a normal green rootstock, grown into a flowering plant in the greenhouse, and crosses were made. The SRI-A15 line was crossed using flowers formed on albino plants grown in sterile culture. Pigment deficiency was maternally inherited in both lines. Physical mapping of the chloroplast genome of the SR1-A15 mutant by SalI, PstI and BamHI restriction endonucleases did not reveal any difference between the SR1-A15 and the parental SRI chloroplast genomes.
Original language | English (US) |
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Pages (from-to) | 637-643 |
Number of pages | 7 |
Journal | Theoretical and Applied Genetics |
Volume | 72 |
Issue number | 5 |
DOIs | |
State | Published - Aug 1986 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
- Genetics
Keywords
- Cell culture
- Nicotiana tabacum
- Physical mapping
- Pigment deficiency
- Plastome mutants