Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency

Z. Svab, P. Maliga

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25 Scopus citations


Callus of Nicotiana tabacum SRI, a mutant with maternally inherited streptomycin resistance, was induced from leaf sections. Callus pieces were mutagenised with N-ethyl-N-nitrosourea and inoculated onto a shoot-induction medium on which calli are normally green. White callus sectors were observed in the mutagenised cultures, and white and variegated shoots were regenerated from these sectored calli. The SR1-A10 line regenerated a chimeric shoot with white leaf margins. The chimeric shoot was grafted onto a normal green rootstock, grown into a flowering plant in the greenhouse, and crosses were made. The SRI-A15 line was crossed using flowers formed on albino plants grown in sterile culture. Pigment deficiency was maternally inherited in both lines. Physical mapping of the chloroplast genome of the SR1-A15 mutant by SalI, PstI and BamHI restriction endonucleases did not reveal any difference between the SR1-A15 and the parental SRI chloroplast genomes.

Original languageEnglish (US)
Pages (from-to)637-643
Number of pages7
JournalTheoretical and Applied Genetics
Issue number5
StatePublished - Aug 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics


  • Cell culture
  • Nicotiana tabacum
  • Physical mapping
  • Pigment deficiency
  • Plastome mutants


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