A nitrite reductase was identified and purified 11 ‐fold from the cytoplasmic fraction of Salmonella typhimurium grown anaerobically with nitrite as the sole nitrogen source. The enzyme required NADH as a cofactor and showed maximum in vitro reductase activity at pH 8.0. S. typhimurium, grown anaerobically in glucose‐limited minimal medium containing peptone and nitrite, showed shorter generation times and increased cell yields in comparison to nitrite‐free cultures. The presence of nitrite had no effect on aerobic cultures. The nitrite reductase functions in a dissimilatory manner and appears to be primarily involved in physiological energy generation during anaerobic growth. The nitrite reductase may function to remove excess reducing power in the form of NADH from the cell thus increasing ATP production during the anaerobic fermentation of glucose. The formation and subsequent assimilation of ammonia from nitrite is of secondary importance to the organism.
|Original language||English (US)|
|Number of pages||13|
|Journal||Journal of Food Safety|
|State||Published - Apr 1985|
All Science Journal Classification (ASJC) codes
- Food Science