Non-invasive transgenic mouse genotyping using stool analysis

Rosemary L. Broome, Li Feng, Qin Zhou, Alicia Smith, Nina Hahn, Suzanne M. Matsui, M. Bishr Omary

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Commonly applied genotyping of transgenic mice involves using tail or ear biopsies which may cause discomfort to the animal. We tested the possibility of polymerase chain reaction (PCR)-based mouse genotyping using stool specimens from three transgenic mouse lines that overexpress 10-18 transgene copies of human keratin polypeptide 18, as compared to genotyping using tail biopsies. Stool specimens were obtained with ease and provided easy detection of the human transgene product. The method was also able to detect endogenous mouse actin and keratin genes which presumably are present at two copies each. Nested PCR was not necessary for genotyping using stool- derived genomic material but did increase the relative magnitude of the signal obtained. The non-invasive genotyping method described herein offers a reproducible, sensitive and effective modality that could replace invasive tissue sampling procedures currently used to test thousands of genetically altered mice.

Original languageEnglish (US)
Pages (from-to)159-160
Number of pages2
JournalFEBS Letters
Volume462
Issue number1-2
DOIs
StatePublished - Nov 26 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Keywords

  • Genotyping
  • Stool analysis
  • Transgenic mouse

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