TY - JOUR
T1 - Nondestructive Characterization of Stem Cell Neurogenesis by a Magneto-Plasmonic Nanomaterial-Based Exosomal miRNA Detection
AU - Lee, Jin Ho
AU - Choi, Jin Ha
AU - Chueng, Sy Tsong Dean
AU - Pongkulapa, Thanapat
AU - Yang, Letao
AU - Cho, Hyeon Yeol
AU - Choi, Jeong Woo
AU - Lee, Ki Bum
N1 - Funding Information:
K.-B.L. acknowledges the partial financial support from the NIH R21 (1R21NS085569 and R21AR071101), NIH R01 (1R01DC016612-01 3R01DC016612-01S1, and 3R01DC016612-02S1), New Jersey Commission on Spinal Cord Research [CSCR17IRG010 and CSCR16ERG019], NSF [CHE-1429062 and CBET-1803517], and the ACS New Directions Award (PRF# 55869-ND10). J.-W.C. acknowledges partial financial support from the NRF (2019R1A2C3002300 and 2016R1A6A1A03012845) funded by MSIP and the ME of Korea. We are also grateful to C. Rathnam, Y. Ying, and K. Kwan for their kind support and valuable discussions on brain explant experiments.
Funding Information:
K.-B.L. acknowledges the partial financial support from the NIH R21 (1R21NS085569 and R21AR071101), NIH R01 (1R01DC016612-01, 3R01DC016612-01S1, and 3R01DC016612-02S1), New Jersey Commission on Spinal Cord Research [CSCR17IRG010 and CSCR16ERG019], NSF [CHE-1429062 and CBET-1803517], and the ACS New Directions Award (PRF# 55869-ND10). J.-W.C. acknowledges partial financial support from the NRF (2019R1A2C3002300 and 2016R1A6A1A03012845) funded by MSIP and the ME of Korea. We are also grateful to C. Rathnam, Y. Ying, and K. Kwan for their kind support and valuable discussions on brain explant experiments.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/8/27
Y1 - 2019/8/27
N2 - The full realization of stem cell-based treatments for neurodegenerative diseases requires precise control and characterization of stem cell fate. Herein, we report a multifunctional magneto-plasmonic nanorod (NR)-based detection platform to address the limitations associated with the current destructive characterization methods of stem cell neurogenesis. Exosomes and their inner contents have been discovered to play critical roles in cell-cell interactions and intrinsic cellular regulations and have received wide attention as next-generation biomarkers. Moreover, exosomal microRNAs (miRNA) also offer an essential avenue for nondestructive molecular analyses of cell cytoplasm components. To this end, our developed nondestructive, selective, and sensitive detection platform has (i) an immunomagnetic active component for exosome isolation and (ii) a plasmonic/metal-enhanced fluorescence component for sensitive exosomal miRNA detection to characterize stem cell differentiation. In a proof-of-concept demonstration, our multifunctional magneto-plasmonic NR successfully detected the expression level of miRNA-124 and characterized neurogenesis of human-induced pluripotent stem cell-derived neural stem cells in a nondestructive and efficient manner. Furthermore, we demonstrated the versatility and feasibility of our multifunctional magneto-plasmonic NRs by characterizing a heterogeneous population of neural cells in an ex vivo rodent model. Collectively, we believe our multifunctional magneto-plasmonic NR-based exosomal miRNA detection platform has a great potential to investigate the function of cell-cell interactions and intrinsic cellular regulators for controlling stem cell differentiation.
AB - The full realization of stem cell-based treatments for neurodegenerative diseases requires precise control and characterization of stem cell fate. Herein, we report a multifunctional magneto-plasmonic nanorod (NR)-based detection platform to address the limitations associated with the current destructive characterization methods of stem cell neurogenesis. Exosomes and their inner contents have been discovered to play critical roles in cell-cell interactions and intrinsic cellular regulations and have received wide attention as next-generation biomarkers. Moreover, exosomal microRNAs (miRNA) also offer an essential avenue for nondestructive molecular analyses of cell cytoplasm components. To this end, our developed nondestructive, selective, and sensitive detection platform has (i) an immunomagnetic active component for exosome isolation and (ii) a plasmonic/metal-enhanced fluorescence component for sensitive exosomal miRNA detection to characterize stem cell differentiation. In a proof-of-concept demonstration, our multifunctional magneto-plasmonic NR successfully detected the expression level of miRNA-124 and characterized neurogenesis of human-induced pluripotent stem cell-derived neural stem cells in a nondestructive and efficient manner. Furthermore, we demonstrated the versatility and feasibility of our multifunctional magneto-plasmonic NRs by characterizing a heterogeneous population of neural cells in an ex vivo rodent model. Collectively, we believe our multifunctional magneto-plasmonic NR-based exosomal miRNA detection platform has a great potential to investigate the function of cell-cell interactions and intrinsic cellular regulators for controlling stem cell differentiation.
KW - exosomal miRNAs
KW - magneto-plasmonic nanorods
KW - neuronal differentiation
KW - nondestructive characterization
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=85071712493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071712493&partnerID=8YFLogxK
U2 - 10.1021/acsnano.9b01875
DO - 10.1021/acsnano.9b01875
M3 - Article
C2 - 31361458
AN - SCOPUS:85071712493
SN - 1936-0851
VL - 13
SP - 8793
EP - 8803
JO - ACS Nano
JF - ACS Nano
IS - 8
ER -