Nondestructive indicator of ethoxyresorufin-o-deethylase activity in embryonic fish

Diane Nacci, Laura Coiro, Anne Kuhn, Denise Champlin, Wayne Munns, Jennifer Specker, Keith Cooper

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Many environmental contaminants of concern produce aryl hydrocarbon (Ah) receptor-mediated effects often assessed as ethoxyresorufin-O-deethylase (EROD) activity. We modified a standardized embryonic and larval fish bioassay using an esmarine fish species (Fundulus heteroclitus) to include measurement of fluorescence in the urinary bladders of living embryos as a nondestructive indicator of EROD activity. This fluorescence was observed only in embryos exposed to the EROD substrate and Ah receptor agonists and was consistent in fluorescence characteristics with the EROD product, resorufin. Aryl hydrocarbon receptoragonist exposures at sublethal concentrations in the parts per billion range, produced significant increases in EROD fluorescence in embryos as young as 3 d postfertilization. These findings are consistent with the conclusion that this fluorescence method is a specific and sensitive nondestructive indicator of Ah receptor-mediated effects in an embryonic fish. As such, this simple method may be useful for assessing effects of environmental contaminants on the early development of fishes and to link molecular indicators with organism-level responses.

Original languageEnglish (US)
Pages (from-to)2481-2486
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume17
Issue number12
DOIs
StatePublished - 1998

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

Keywords

  • Aryl hydrocarbon receptor-mediated effects
  • Embryonic development
  • Ethoxyresorufin-O- deethylase
  • Fish bioassay
  • Fundulus heteroclitus

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