TY - JOUR
T1 - Nondestructive Real-Time Monitoring of Enhanced Stem Cell Differentiation Using a Graphene-Au Hybrid Nanoelectrode Array
AU - Lee, Jin Ho
AU - Choi, Hye Kyu
AU - Yang, Letao
AU - Chueng, Sy Tsong Dean
AU - Choi, Jeong Woo
AU - Lee, Ki Bum
N1 - Funding Information:
K.B.L. acknowledges the partial financial support from the NIH R21 (1R21AR071101-01), the NSF (CHE-1429062), and New Jersey Commission on Spinal Cord (CSCR16ERG019). J.W.C. acknowledges partial financial support from the NRF (2013K1A4A3055268) and (2016R1A6A1A03012845) funded by MSIP and the ME of Korea. The authors are also grateful to Thanapat Pongkulapa and Dr. Tae-Hyung Kim for their kind support and valuable discussion.
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/9/26
Y1 - 2018/9/26
N2 - Stem cells have attracted increasing research interest in the field of regenerative medicine because of their unique ability to differentiate into multiple cell lineages. However, controlling stem cell differentiation efficiently and improving the current destructive characterization methods for monitoring stem cell differentiation are the critical issues. To this end, multifunctional graphene–gold (Au) hybrid nanoelectrode arrays (NEAs) to: (i) investigate the effects of combinatorial physicochemical cues on stem cell differentiation, (ii) enhance stem cell differentiation efficiency through biophysical cues, and (iii) characterize stem cell differentiation in a nondestructive real-time manner are developed. Through the synergistic effects of physiochemical properties of graphene and biophysical cues from nanoarrays, the graphene-Au hybrid NEAs facilitate highly enhanced cell adhesion and spreading behaviors. In addition, by varying the dimensions of the graphene-Au hybrid NEAs, improved stem cell differentiation efficiency, resulting from the increased focal adhesion signal, is shown. Furthermore, graphene-Au hybrid NEAs are utilized to monitor osteogenic differentiation of stem cells electrochemically in a nondestructive real-time manner. Collectively, it is believed the unique multifunctional graphene-Au hybrid NEAs can significantly advance stem-cell-based biomedical applications.
AB - Stem cells have attracted increasing research interest in the field of regenerative medicine because of their unique ability to differentiate into multiple cell lineages. However, controlling stem cell differentiation efficiently and improving the current destructive characterization methods for monitoring stem cell differentiation are the critical issues. To this end, multifunctional graphene–gold (Au) hybrid nanoelectrode arrays (NEAs) to: (i) investigate the effects of combinatorial physicochemical cues on stem cell differentiation, (ii) enhance stem cell differentiation efficiency through biophysical cues, and (iii) characterize stem cell differentiation in a nondestructive real-time manner are developed. Through the synergistic effects of physiochemical properties of graphene and biophysical cues from nanoarrays, the graphene-Au hybrid NEAs facilitate highly enhanced cell adhesion and spreading behaviors. In addition, by varying the dimensions of the graphene-Au hybrid NEAs, improved stem cell differentiation efficiency, resulting from the increased focal adhesion signal, is shown. Furthermore, graphene-Au hybrid NEAs are utilized to monitor osteogenic differentiation of stem cells electrochemically in a nondestructive real-time manner. Collectively, it is believed the unique multifunctional graphene-Au hybrid NEAs can significantly advance stem-cell-based biomedical applications.
KW - biosensing
KW - graphene-Au hybrid nanoelectrode arrays
KW - nondestructive real-time detection
KW - osteogenesis from stem cells
KW - stem cell differentiation
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U2 - 10.1002/adma.201802762
DO - 10.1002/adma.201802762
M3 - Article
C2 - 30073706
AN - SCOPUS:85052474742
SN - 0935-9648
VL - 30
JO - Advanced Materials
JF - Advanced Materials
IS - 39
M1 - 1802762
ER -