Novel high-level expression cloning vehicles: 104-Fold amplification of esiherkhia coll minor protein

Yoshihiro Masui, Takeshi Mizuno, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Versatile, high-level expression cloning vehicles, pINIII, have been constructed. A foreign DNA fragment can be inserted in any one of the three reading frames at the unique EcoRI, HindIII, or BamHI site immediately after the initiation codon. The cloned foreign gene is under the control of both the lpp promoter and the lac promoter-operator. The expression of the gene is regulated by the lac repressor produced by the same vehicles. Using a pINIII vehicle EnvZ protein, a very minor regulatory protein of Escherichia coli was amplified more than 104-fold, becoming 30% of total cellular protein.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalBio/Technology
Volume2
Issue number1
DOIs
StatePublished - Jan 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology

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