TY - JOUR
T1 - NS-4 (nervous system antigen-4), a cell surface antigen of developing and adult mouse brain and sperm
AU - Schachner, Melitta
AU - Wortham, Kathleen A.
AU - Carter, Louvenia D.
AU - Chaffee, Jonathan K.
N1 - Funding Information:
The authors are indebted to Dr. Richard L. Sid-man for his valuable comments on the manuscript. This research was supported by the Alfred P. Sloan Foundation, The National Science Foundation (Grant No. GB-34186) and the National Institutes of Health (Training Grants No. TO1 HD00415 and MH 07084-14).
PY - 1975/6
Y1 - 1975/6
N2 - When rabbits are injected with particulate fractions of cerebellum from 4-day-old C57BL 6J mice, an antiserum is produced that is cytotoxic for single-cell suspensions from cerebellum. After several absorptions with nonnervous system-derived tissues (liver, spleen, kidney, thymocytes) this heterologous antiserum reacts only with tissue from the developing and adult nervous system and sperm from vas deferens and epididymis. This antigen or set of antigens is present in neuroectodermally derived tissues at all ages tested: 12-day-old embryos and 12-month-old adults being the earliest and latest stages, respectively. The antigen is expressed not only on cerebellum but also on all parts of the normal central nervous system tested including retina. It is demonstrable on a murine medulloepithelioma and to a lesser degree on glioblastoma and ependymoblastoma but not on glioma G26, ependymoblastoma EPA and neuroblastoma C1300. The antigen is found in all mouse strains tested ( C57BL 6J, ICR, BALB cJ, A J and ( C57BL 6J × C3H HeJ) F1 hybrid) and in rat.
AB - When rabbits are injected with particulate fractions of cerebellum from 4-day-old C57BL 6J mice, an antiserum is produced that is cytotoxic for single-cell suspensions from cerebellum. After several absorptions with nonnervous system-derived tissues (liver, spleen, kidney, thymocytes) this heterologous antiserum reacts only with tissue from the developing and adult nervous system and sperm from vas deferens and epididymis. This antigen or set of antigens is present in neuroectodermally derived tissues at all ages tested: 12-day-old embryos and 12-month-old adults being the earliest and latest stages, respectively. The antigen is expressed not only on cerebellum but also on all parts of the normal central nervous system tested including retina. It is demonstrable on a murine medulloepithelioma and to a lesser degree on glioblastoma and ependymoblastoma but not on glioma G26, ependymoblastoma EPA and neuroblastoma C1300. The antigen is found in all mouse strains tested ( C57BL 6J, ICR, BALB cJ, A J and ( C57BL 6J × C3H HeJ) F1 hybrid) and in rat.
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U2 - 10.1016/0012-1606(75)90402-9
DO - 10.1016/0012-1606(75)90402-9
M3 - Article
C2 - 48485
AN - SCOPUS:0016761281
VL - 44
SP - 313
EP - 325
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -