Nuclear localization of prostaglandin E2 receptors

Mousumi Bhattacharya, Krishna G. Peri, Guillermina Almazan, Alfredo Ribeiro-Da-Silva, Hitoshi Shichi, Yves Durocher, Mark Abramovitz, Xin Hou, Daya R. Varma, Sylvain Chemtob

Research output: Contribution to journalArticlepeer-review

210 Scopus citations


Prostaglandin E2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP1 and in human embryonic kidney 293 (Epstein-Barr virus-encoded nuclear antigen) cells expressing EP1 fused to green fluorescent protein. High- resolution immunostaining of EP1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.

Original languageEnglish (US)
Pages (from-to)15792-15797
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number26
StatePublished - Dec 22 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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