Nucleic acid extraction using a rapid, chemical free, ultrasonic technique for point-of-care diagnostics

Darren W. Branch, Gennifer T. Smith, Erika C. Vreeland, Robert Blakemore, David Alland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


We have developed a miniature ultrasonic lysing system for the rapid release and extraction of Mycobacterium Tuberculosis (MTB) DNA. The prototype consists of two key sections, a disposable plastic manifold that hold sputum slides and a plastic cartridge with five fluidic DNA extraction channels. The MTB vaccine Bacillus Calmette-Guérin (BCG) was used as a simulant for MTB for all the lysing studies. The slide manifold houses up to five individual sputum slides and is processed by a computer to fill and load the cartridges with sputum suspensions from each slide. The plastic cartridge was developed to facilitate acoustic coupling through the bottom interface such that MTB/BCG can be lysed remotely. The disposable cartridge eliminated cross contamination problems and additional cleaning time. Due to the high power density, only 66 mW was required to lyse microliter BCG samples at the same level as the positive control (i.e. boiling for 30 minutes at 95 °C).

Original languageEnglish (US)
Title of host publicationIEEE International Ultrasonics Symposium, IUS
PublisherIEEE Computer Society
Number of pages6
ISBN (Electronic)9781479970490
StatePublished - Oct 20 2014
Event2014 IEEE International Ultrasonics Symposium, IUS 2014 - Chicago, United States
Duration: Sep 3 2014Sep 6 2014

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727


Other2014 IEEE International Ultrasonics Symposium, IUS 2014
Country/TerritoryUnited States

All Science Journal Classification (ASJC) codes

  • Acoustics and Ultrasonics


  • Mycobacterium tuberculosis formatting
  • PCR
  • Point-of-Care
  • acoustic lysis


Dive into the research topics of 'Nucleic acid extraction using a rapid, chemical free, ultrasonic technique for point-of-care diagnostics'. Together they form a unique fingerprint.

Cite this