Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Rotem Ben Tov Perry; Ida Rishal; Ella Doron-Mandel; Ashley L. Kalinski; Katalin F. Medzihradszky; Marco Terenzio; Stefanie Alber; Sandip Koley; Albina Lin; Meir Rozenbaum; Dmitry Yudin; Pabitra K. Sahoo; Cynthia Gomes; Vera Shinder; Wasim Geraisy; Eric A. Huebner; Clifford J. Woolf; Avraham Yaron; Alma L. Burlingame; Jeffery L. Twiss; Mike Fainzilber

doi:10.1016/j.celrep.2016.07.005

Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Rotem Ben Tov Perry, Ida Rishal, Ella Doron-Mandel, Ashley L. Kalinski, Katalin F. Medzihradszky, Marco Terenzio, Stefanie Alber, Sandip Koley, Albina Lin, Meir Rozenbaum, Dmitry Yudin, Pabitra K. Sahoo, Cynthia Gomes, Vera Shinder, Wasim Geraisy, Eric A. Huebner, Clifford J. Woolf, Avraham Yaron, Alma L. Burlingame, Jeffery L. TwissMike Fainzilber

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin β1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin β1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin β1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

Original language	English (US)
Pages (from-to)	1664-1676
Number of pages	13
Journal	Cell Reports
Volume	16
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2016.07.005
State	Published - Aug 9 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2016.07.005

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Perry, R. B. T., Rishal, I., Doron-Mandel, E., Kalinski, A. L., Medzihradszky, K. F., Terenzio, M., Alber, S., Koley, S., Lin, A., Rozenbaum, M., Yudin, D., Sahoo, P. K., Gomes, C., Shinder, V., Geraisy, W., Huebner, E. A., Woolf, C. J., Yaron, A., Burlingame, A. L., ... Fainzilber, M. (2016). Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size. Cell Reports, 16(6), 1664-1676. https://doi.org/10.1016/j.celrep.2016.07.005

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title = "Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size",

abstract = "How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin β1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin β1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin β1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.",

author = "Perry, {Rotem Ben Tov} and Ida Rishal and Ella Doron-Mandel and Kalinski, {Ashley L.} and Medzihradszky, {Katalin F.} and Marco Terenzio and Stefanie Alber and Sandip Koley and Albina Lin and Meir Rozenbaum and Dmitry Yudin and Sahoo, {Pabitra K.} and Cynthia Gomes and Vera Shinder and Wasim Geraisy and Huebner, {Eric A.} and Woolf, {Clifford J.} and Avraham Yaron and Burlingame, {Alma L.} and Twiss, {Jeffery L.} and Mike Fainzilber",

note = "Publisher Copyright: {\textcopyright} 2016 The Author(s)",

year = "2016",

month = aug,

day = "9",

doi = "10.1016/j.celrep.2016.07.005",

language = "English (US)",

volume = "16",

pages = "1664--1676",

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Perry, RBT, Rishal, I, Doron-Mandel, E, Kalinski, AL, Medzihradszky, KF, Terenzio, M, Alber, S, Koley, S, Lin, A, Rozenbaum, M, Yudin, D, Sahoo, PK, Gomes, C, Shinder, V, Geraisy, W, Huebner, EA, Woolf, CJ, Yaron, A, Burlingame, AL, Twiss, JL & Fainzilber, M 2016, 'Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size', Cell Reports, vol. 16, no. 6, pp. 1664-1676. https://doi.org/10.1016/j.celrep.2016.07.005

TY - JOUR

T1 - Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

AU - Perry, Rotem Ben Tov

AU - Rishal, Ida

AU - Doron-Mandel, Ella

AU - Kalinski, Ashley L.

AU - Medzihradszky, Katalin F.

AU - Terenzio, Marco

AU - Alber, Stefanie

AU - Koley, Sandip

AU - Lin, Albina

AU - Rozenbaum, Meir

AU - Yudin, Dmitry

AU - Sahoo, Pabitra K.

AU - Gomes, Cynthia

AU - Shinder, Vera

AU - Geraisy, Wasim

AU - Huebner, Eric A.

AU - Woolf, Clifford J.

AU - Yaron, Avraham

AU - Burlingame, Alma L.

AU - Twiss, Jeffery L.

AU - Fainzilber, Mike

PY - 2016/8/9

Y1 - 2016/8/9

N2 - How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin β1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin β1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin β1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

AB - How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin β1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin β1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin β1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

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EP - 1676

JO - Cell Reports

JF - Cell Reports

IS - 6

ER -

Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

Abstract

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