Nucleotide sequence of RNA2 and polyprotein processing sites of a Chinese isolate of broad bean wilt virus

Yijun Qi, Xueping Zhou, Chaoyang Xue, Debao Li

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

RNA2 of broad bean wilt virus 2 (BBWV2) isolate B935 is composed of 3601 nucleotide (nt) residues, exclusive of the polyadenylate at the 3' end. Only one of the six possible reading frames has a long open reading frame, which extends from nt 231 to nt 3428 in the polarity of encapsidated RNA, and encodes a polyprotein of 119 kD. The N-terminus of the large coat protein (LCP) is located at 599 nt and the small coat protein (SCP) at 197 nt from the C-terminus of the 119 kD protein, which suggests that the coat proteins are released from the polyprotein by cleavages of a glutamine-glycine (Q-G) and a glutamine-alanine (Q-A) bond respectively. The sequence comparison of B935 with fabaviruses shows that B935 has very high sequence homology with other BBWY2 isolates and with patchouli mild mosaic virus, but has lower homology with BBWV1 isolates. B935 has a similar genomic organization, but a low sequence homology to RNA2 molecules of comoviruses and nepoviruses.

Original languageEnglish (US)
Pages (from-to)684-686
Number of pages3
JournalProgress in Natural Science
Volume10
Issue number9
StatePublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Keywords

  • Broad bean wilt virus
  • Polyprotein processing sites
  • RNA2 sequence

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