Nutritional control of fatty acid esterification in differentiating Caco- 2 intestinal cells is mediated by cellular diacylglycerol concentrations

P. J. Trotter, J. Storch

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The Caco-2 human intestinal cell line was used to investigate the effects of exogenous lipid on fatty acid esterification in differentiating intestinal absorptive cells. Preincubation of Caco-2 cells with either palmitate or palmitate plus 2-monoolein resulted in greater utilization of subsequently added fatty acid for triacylglycerol relative to phosphatidylcholine synthesis. Despite this lipid-induced alteration in metabolism, the activities of acyl-CoA synthetase, glycerol-3-phosphate acyltransferase and diacylglycerol acyltransferase were unchanged. In addition, monoacylglycerol acyltransferase activity was nearly undetectable, even after preincubation with 2-monoolein. The intracellular diacylglycerol concentrations were, however, increased with greater lipid substrate availability. These studies indicate that, under conditions of increased dietary lipid, intestinal fatty acid esterification via the glycerol-3-phosphate pathway is modulated by cellular diacylglycerol concentrations.

Original languageEnglish (US)
Pages (from-to)728-736
Number of pages9
JournalJournal of Nutrition
Volume123
Issue number4
DOIs
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Keywords

  • enzymes
  • fatty acids
  • intestinal epithelial cells
  • lipid

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