O-glycoside orientation is an essential aspect of base J recognition by the kinetoplastid DNA-binding protein JBP1

Rajesh K. Grover, Stephanie J.K. Pond, Qizhi Cui, Prem Subramaniam, David A. Case, David P. Millar, Paul Wentworth

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A binding edge? Comparison of dissociation constants and molecular-dynamics snapshots of a panel of synthetic telomeric double-stranded DNA sequences containing HMdU O-glycosides has revealed that the DNA-binding protein of kinetoplastids, such as Leishmania and Trypanosoma, JBP1, recognizes a critical edge-on conformation of the pyranosyl ring of base J. If this conformation is perturbed, JBP1 binding is dramatically reduced.(Chemical Equation Presented).

Original languageEnglish (US)
Pages (from-to)2839-2843
Number of pages5
JournalAngewandte Chemie - International Edition
Volume46
Issue number16
DOIs
StatePublished - 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • DNA
  • Glycosides
  • Molecular dynamics
  • Molecular recognition

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