Open neutral tube defects: Immunocytochemical demonstration of neuroepithelial cells in amniotic fluid

Ellen Greenebaum, Mahesh M. Mansukhani, Debra Heller, Ilan Timor-Tristsch

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytologic evaluation of second trimester amniotic fluid (AF) is a rapid, inexpensive adjunct to prenatal diagnosis of open neural tube defects (ONTDs). Our goal was to determine whether the neural-appearing cells and/or large foamy macrophages in the AF of anencephalics are indeed of neural and/or glial origin. In two second trimester patients with elevated serum alpha-fetoprotein (AFP) and polyhydramnios, fetal sonogram studies showed anencephaly; amniocentesis was performed for AF-AFP, cytogenetic, and cytologic studies. AF sediment smears were initially Papanicolaou-stained; next, the same smears were immunoperoxidase (IP)-stained for glial fibrillary acidic protein (GFAP). If GFAP negative, slides were restrained for synaptophysin (SYN) and neuron-specific enolase (NSE). Both AFs contained small neural-appearing cells (5-10 μm) singly and in clusters, with dense, round, homogenous nuclei, an occasional nucleolus, and scant cytoplasmic rim. These were GFAP negative and SYN and NSE positive; the large vacuolated, lipid-laden macrophages (20-40 μm) were negative for all three IP stains. In conclusion, positive IP staining for SYN and NSE supports the morphologic impression that small dark cells in AF are of neural origin, while negative IP staining of large foamy macrophages suggests nonneural, nonglial origin.

Original languageEnglish (US)
Pages (from-to)143-144
Number of pages2
JournalDiagnostic Cytopathology
Volume16
Issue number2
DOIs
StatePublished - Feb 1 1997
Externally publishedYes

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Neuroepithelial Cells
Amniotic Fluid
Synaptophysin
Phosphopyruvate Hydratase
Glial Fibrillary Acidic Protein
Macrophages
alpha-Fetoproteins
Second Pregnancy Trimester
Anencephaly
Polyhydramnios
Negative Staining
Amniocentesis
Neural Tube Defects
Prenatal Diagnosis
Cytogenetics
Neuroglia
Coloring Agents
Staining and Labeling
Lipids
Serum

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

Greenebaum, Ellen ; Mansukhani, Mahesh M. ; Heller, Debra ; Timor-Tristsch, Ilan. / Open neutral tube defects : Immunocytochemical demonstration of neuroepithelial cells in amniotic fluid. In: Diagnostic Cytopathology. 1997 ; Vol. 16, No. 2. pp. 143-144.
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Open neutral tube defects : Immunocytochemical demonstration of neuroepithelial cells in amniotic fluid. / Greenebaum, Ellen; Mansukhani, Mahesh M.; Heller, Debra; Timor-Tristsch, Ilan.

In: Diagnostic Cytopathology, Vol. 16, No. 2, 01.02.1997, p. 143-144.

Research output: Contribution to journalArticle

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