Optimal integration of wide field illumination and holographic optical tweezers for multimodal microscopy with ultimate flexibility and versatility

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Abstract

We introduce one-of-a-kind optical microscope that we have developed through optimized integration of wide-field and focused-light microscopies. This new instrument has accomplished operation of the same laser for both wide field illumination and holographic focused beam illumination interchangeably or simultaneously in a way scalable to multiple lasers. We have demonstrated its powerful capability by simultaneously carrying out Epi-fluorescence, total internal reflection fluorescence microscopy, selective plane illumination microscopy, and holographic optical tweezers with five lasers. Our instrument and the optical design will provide researchers across diverse fields, cell-biology and biophysics in particular, with a practical guidance to build an all-around multimodal microscope that will further inspire the development of novel hybrid microscopy experiments.

Original languageEnglish (US)
Pages (from-to)8049-8058
Number of pages10
JournalOptics Express
Volume26
Issue number7
DOIs
StatePublished - Apr 2 2018

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versatility
flexibility
illumination
microscopy
lasers
biophysics
fluorescence
optical microscopes
microscopes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

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abstract = "We introduce one-of-a-kind optical microscope that we have developed through optimized integration of wide-field and focused-light microscopies. This new instrument has accomplished operation of the same laser for both wide field illumination and holographic focused beam illumination interchangeably or simultaneously in a way scalable to multiple lasers. We have demonstrated its powerful capability by simultaneously carrying out Epi-fluorescence, total internal reflection fluorescence microscopy, selective plane illumination microscopy, and holographic optical tweezers with five lasers. Our instrument and the optical design will provide researchers across diverse fields, cell-biology and biophysics in particular, with a practical guidance to build an all-around multimodal microscope that will further inspire the development of novel hybrid microscopy experiments.",
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