Optimized 3d culture of hepatic cells for liver organoid metabolic assays

Christian Moya Gamboa, Yujue Wang, Huiting Xu, Katarzyna Kalemba, Fredric E. Wondisford, Hatem E. Sabaawy

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The liver is among the principal organs for glucose homeostasis and metabolism. Studies of liver metabolism are limited by the inability to expand primary hepatocytes in vitro while maintaining their metabolic functions. Human hepatic three-dimensional (3D) organoids have been established using defined factors, yet hepatic organoids from adult donors showed impaired expan-sion. We examined conditions to facilitate the expansion of adult donor-derived hepatic organoids (HepAOs) and HepG2 cells in organoid cultures (HepGOs) using combinations of growth factors and small molecules. The expansion dynamics, gluconeogenic and HNF4α expression, and albumin secretion are assessed. The conditions tested allow the generation of HepAOs and HepGOs in 3D cultures. Nevertheless, gluconeogenic gene expression varies greatly between conditions. The organoid expansion rates are limited when including the TGFβ inhibitor A8301, while are relatively higher with Forskolin (FSK) and Oncostatin M (OSM). Notably, expanded HepGOs grown in the optimized condition maintain detectable gluconeogenic expression in a spatiotemporal distribution at 8 weeks. We present optimized conditions by limiting A8301 and incorporating FSK and OSM to allow the expansion of HepAOs from adult donors and HepGOs with gluconeogenic compe-tence. These models increase the repertoire of human hepatic cellular tools available for use in liver metabolic assays.

Original languageEnglish (US)
Article number3280
JournalCells
Volume10
Issue number12
DOIs
StatePublished - Dec 2021

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Keywords

  • 3D culture
  • Gluconeogenesis
  • HepG2 cells
  • Liver regeneration
  • Metabolic assays
  • Organoids

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