Orai1 plays a crucial role in central sensitization by modulating neuronal excitability

Yannong Dou, Jingsheng Ia, Ruby Gao, Inghua Gao, Frances M. Munoz, Dongyu Wei, Yuzhen Tian, James E. Barrett, Seena Ajit, Olimpia Meucci, James W. Putney, Yue Dai, Huijuan Hu

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Pathological pain is a common and debilitating condition that is often poorly managed. Central sensitization is an important mechanism underlying pathological pain. However, candidate molecules involved in central sensitization remain unclear. Store-operated calcium channels (SOCs) mediate important calcium signals in nonexcitable and excitable cells. SOCs have been implicated in a wide variety of human pathophysiological conditions, including immunodeficiency, occlusive vascular diseases, and cancer. However, the role of SOCs in CNS disorders has been relatively unexplored. Orai1, a key component of SOCs, is expressed in the human and rodent spinal cord dorsal horn, but its functional significance in dorsal horn neurons is poorly understood. Here we sought to explore a potential role of Orai1 in the modulation of neuronal excitability and A-type potassium channels involved in pain plasticity. Using both male and female Orai1 knock-out mice, we found that activation of Orai1 increased neuronal excitability and reduced A-type potassium channels via the protein kinase C–extracellular signal-regulated protein kinase (PKC–ERK) pathway in dorsal horn neurons. Orai1 deficiency significantly decreased acute pain induced by noxious stimuli, nearly eliminated the second phase of formalin-induced nociceptive response, markedly attenuated carrageenan-induced ipsilateral pain hypersensitivity and abolished carrageenan-induced contralateral mechanical allodynia. Consistently, carrageenan-induced increase in neuronal excitability was abolished in the dorsal horn from Orai1 mutant mice. These findings uncover a novel signaling pathway involved in the pain process and central sensitization. Our study also reveals a novel link among Orai1, ERK, A-type potassium channels, and neuronal excitability.

Original languageEnglish (US)
Pages (from-to)887-900
Number of pages14
JournalJournal of Neuroscience
Issue number4
StatePublished - Jan 24 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


  • A-type potassium channels
  • ERK
  • Orai1
  • Pain
  • Spinal cord dorsal horn
  • Store-operated calcium channels


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