TY - JOUR
T1 - Origin and mode of replication of plasmids pE194 and pUB110
AU - Scheer-Abramowitz, J.
AU - Gryczan, T. J.
AU - Dubnau, D.
N1 - Funding Information:
We thank I. Smith, E. Dubnau, and R. P. Novick for useful discussions, J. Poindexter and T. Broker for advice on electron microscopy, J. Hagenzieker for technical assistance, and A. Howard for expert secretarial assistance. This work was supported by USPHS Grant AI-1031 1 and American Cancer Society Grant VC300 awarded to D.D.
PY - 1981/7
Y1 - 1981/7
N2 - Replication of the multicopy antibiotic resistance plasmids pE194 and pUB110 has been studied in Bacillus subtilis. Based on electron microscopic analysis of replication intermediates and on evidence from deletants, we have determined that both plasmids replicate unidirectionally from fixed origins by a Θ mechanism. The location of these origins and the directions of replication have been determined relative to the physical maps. A cointegrate of pE194 and pUB110 has also been studied and evidence is presented from electron microscopy, temperature resistance, and copy number analysis that the cointegrate uses both parental replication origins.
AB - Replication of the multicopy antibiotic resistance plasmids pE194 and pUB110 has been studied in Bacillus subtilis. Based on electron microscopic analysis of replication intermediates and on evidence from deletants, we have determined that both plasmids replicate unidirectionally from fixed origins by a Θ mechanism. The location of these origins and the directions of replication have been determined relative to the physical maps. A cointegrate of pE194 and pUB110 has also been studied and evidence is presented from electron microscopy, temperature resistance, and copy number analysis that the cointegrate uses both parental replication origins.
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U2 - 10.1016/0147-619X(81)90054-8
DO - 10.1016/0147-619X(81)90054-8
M3 - Article
C2 - 6269131
AN - SCOPUS:0019373310
SN - 0147-619X
VL - 6
SP - 67
EP - 77
JO - Plasmid
JF - Plasmid
IS - 1
ER -