The calcineurin B-like (CBL) proteins, comprising a large subfamily of calcium sensors in plant cells, play an important role in many stress responses. We cloned a gene from the halophyte Thellungiella halophila that is homologous to AtCBL9 in Arabidopsis thaliana. The 1008-bp ThCBL9 contains an ORF of 639 bp and encodes 213 amino acids, with a 5′-untranslated region of 193 bp and a 3′-untranslated region of 176 bp. Its amino acid sequence shares high homology with AtCBLs. ThCBL9 is upregulated by ABA, NaCl, and PEG in Thellungiella leaves. Using molecular biological methods, we over-expressed ThCBL9 in A. thaliana and found that this enhanced tolerances to both high salt and osmotic stress in transgenic Arabidopsis.
All Science Journal Classification (ASJC) codes
- Plant Science
- Stress tolerance
- Thellungiella halophila