Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells

S. E. Pories, D. T. Hess, K. Swenson, M. Lotz, R. Moussa, Jr Steele G., D. Shibata, K. M. Rieger-Christ, I. C. Summerhayes

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Background and Aims: Src activation is reported as an early event found in preneoplastic colonic adenomas and in 70% of colon carcinomas. The aim of this study was to identify the biological consequences of c-src overexpression in rat colon epithelial cells. Methods: Introduction and overexpression of c-src in an immortalized rat colon epithelial cell line was achieved using lipofection. Transfectants were tested for changes in growth and cell behavior using different in vitro assay systems. Results: Colon epithelial cells overexpressing c-src showed the ability to form microcolonies in soft agar without acquiring tumorigenic potential. In in vitro assays, c-src transfectants displayed a gain of invasive potential through Matrigel without an accompanying change in migrational ability. No discernible qualitative changes were observed in the phosphotyrosyl protein profile between c-src and v-src transfectants. Assessment of the cadherin/catenin status in these cells revealed an intact, functional complex with no detectable tyrosine phosphorylation of different components of the complex. Conclusions: Overexpression of c-src in an immortalized rat colon epithelial cell line does not elicit full neoplastic transformation but enhances anchorage-independent growth and confers invasion capability. Increased invasion through Matrigel was not linked to inactivation of the cadherin complex in c-src transfectants.

Original languageEnglish (US)
Pages (from-to)1287-1295
Number of pages9
JournalGastroenterology
Volume114
Issue number6
DOIs
StatePublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

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