Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation

Shida Yousefi, Satish K. Sharma, Darko Stojkov, Nina Germic, Salome Aeschlimann, Moyar Q. Ge, Cameron H. Flayer, Erik D. Larson, Imre G. Redai, Suhong Zhang, Cynthia J. Koziol-White, Katalin Karikó, Hans Uwe Simon, Angela Haczku

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The asthmatic airways are highly susceptible to inflammatory injury by air pollutants such as ozone (O 3 ), characterized by enhanced activation of eosinophilic granulocytes and a failure of immune protective mechanisms. Eosinophil activation during asthma exacerbation contributes to the proinflammatory oxidative stress by high levels of nitric oxide (NO) production and extracellular DNA release. Surfactant protein-D (SP-D), an epithelial cell product of the airways, is a critical immune regulatory molecule with a multimeric structure susceptible to oxidative modifications. Using recombinant proteins and confocal imaging, we demonstrate here that SP-D directly bound to the membrane and inhibited extracellular DNA trap formation by human and murine eosinophils in a concentration and carbohydrate-dependent manner. Combined allergic airway sensitization and O 3 exposure heightened eosinophilia and nos2 mRNA (iNOS) activation in the lung tissue and S-nitrosylation related de-oligomerisation of SP-D in the airways. In vitro reproduction of the iNOS action led to similar effects on SP-D. Importantly, S-nitrosylation abolished the ability of SP-D to block extracellular DNA trap formation. Thus, the homeostatic negative regulatory feedback between SP-D and eosinophils is destroyed by the NO-rich oxidative lung tissue environment in asthma exacerbations.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalJournal of Leukocyte Biology
Volume104
Issue number1
DOIs
StatePublished - Jul 2018

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Keywords

  • eosinophil extracellular traps (EETs)
  • eosinophils
  • surfactant pulmonary-associated protein D (SP-D)

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