p53/56(lyn) antisense shifts the 1,25-dihydroxyvitamin D3-induced G1/S block in HL60 cells to S phase

Qing Mei Wang, George P. Studzinski, Fei Chen, Frederick D. Coffman, Lawrence E. Harrison

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


p53/56(lyn) is a member of the src family that is predominantly expressed in hematopoietic cells and is thought to play a role in cellular proliferation. In this study, we demonstrate the participation of p53/56(lyn) in 1,25-dihydroxyvitamin D3 (1,25D3)-induced growth arrest in HL60 cells. We show that the mRNA and protein levels of p53/56(lyn) are markedly elevated after 1,25D3 treatment, which is accompanied by an increase of p53/56(lyn) kinase activity. We also demonstrate that treatment with p53/56(lyn) antisense oligodeoxynucleotides reverses the 1,25D3-induced G1/S block, and results in an accumulation of cells with S-phase DNA content. BrdU pulse- chase experiments reveal that this accumulation results from an increased proportion of cells actively synthesizing DNA, which are inhibited from exiting the S-phase compartment. These results indicate that upregulation of p53/56(lyn) contributes significantly to the G1/S growth arrest induced by 1,25D3 in HL60 cells and thus its activation may be a desirable outcome of chemotherapeutic regimens. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)238-246
Number of pages9
JournalJournal of Cellular Physiology
Issue number2
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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