Packaging, replication and recombination of the segmented genomes of bacteriophage Φ6 and its relatives

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Abstract

The genomes of bacteriophage Φ6 and its relatives are packaged through a mechanism that involves the recognition and translocation of the three different plus strand transcripts of the segmented dsRNA genomes into preformed polyhedral structures called procapsids or inner cores. The packaging requires hydrolysis of NTPs and takes place in the order S:M:L. Minus strand synthesis begins after the completion of the plus strand packaging. The packaging and replication reactions can be studied in vitro with purified components. A model has been presented that proposes that the program of serially dependent packaging is determined by the conformational changes at the surface of the procapsid due to the amount of RNA packaged at each step. The in vitro packaging and replication system has facilitated the application of reverse genetics and the study of recombination in the family of Cystoviridae.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalVirus Research
Volume101
Issue number1
DOIs
StatePublished - Apr 2004

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases

Keywords

  • Bacteriophage
  • Genomic packaging
  • Pseudomonas
  • dsRNA

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