Penicillium species endophytic in coffee plants and ochratoxin A production

Fernando E. Vega, Francisco Posada, Stephen W. Peterson, Thomas J. Gianfagna, Fabio Chaves

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Tissues from Coffea arabica, C. congensis, C. dewevrei and C. liberica collected in Colombia, Hawaii and at a local plant nursery in Maryland were sampled for the presence of fungal endophytes. Surface sterilized tissues including roots, leaves, stems and various berry parts were plated on yeast-malt agar. DNA was extracted from a set of isolates visually recognized as Penicillium, and the internal transcribed spacer region and partial LSU-rDNA was amplified and sequenced. Comparison of DNA sequences with GenBank and unpublished sequences revealed the presence of 11 known Penicillium species: P. brevicompactum, P. brocae, P. cecidicola, P. citrinum, P. coffeae, P. crustosum, P. janthinellum, P. olsonii, P. oxalicum, P. sclerotiorum and P. steckii as well as two possibly undescribed species near P. diversum and P. roseopurpureum. Ochratoxin A was produced by only four isolates, one isolate each of P. brevicompactum, P. crustosum, P. olsonii and P. oxalicum. The role these endophytes play in the biology of the coffee plant remains enigmatic.

Original languageEnglish (US)
Pages (from-to)31-42
Number of pages12
JournalMycologia
Volume98
Issue number1
DOIs
StatePublished - 2006

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

Keywords

  • Coffea arabica
  • Coffea congensis
  • Coffea dewevrei
  • Coffea liberica
  • Endophytes
  • OTA

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