Permeation of dimyristoylphosphatidylcholine into skin - Structural and spatial information from IR and Raman microscopic imaging

Chunhong Xiao, David J. Moore, Carol R. Flach, Richard Mendelsohn

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The permeation of size fractionated acyl-chain perdeuterated 1,2-dimyristoylphosphatidylcholine (DMPC-d54) into pigskin was monitored by infrared microscopic imaging and by confocal Raman microscopy. The former technique permits the examination of the relative concentration of molecular species (e.g. endogenous and exogenous lipids and proteins) over comparatively large spatial areas (∼1-10 mm) with a spatial resolution of ∼10-12 μm. Raman microscopy permits the confocal examination of tissue at depths up to 50 μm with a pixel size of about 2-3 μm3. However, Raman signal/noise levels are reduced from IR and significantly smaller areas are routinely sampled conveniently. DMPC-d54 permeated ∼25-35 μm, with most of the exogenous lipid residing in the stratum corneum, a result generally in accord with literature values. In addition, the state of the DMPC-d54 (intact vesicles or molecularly dispersed with skin constituents) was evaluated from the spatial dependence of the perdeuterated myristate chain conformational order. Uncertainties in the measurement of permeation distances by vibrational spectroscopy are noted.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalVibrational Spectroscopy
Volume38
Issue number1-2
DOIs
StatePublished - Jul 29 2005

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Keywords

  • Drug delivery
  • IR imaging
  • Liposome permeation
  • Raman imaging
  • Stratum corneum
  • Transdermal

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