The authors conducted in vitro studies on the interaction of red blood cells (RBC) from Rauscher leukemia virus (RLV)-infected mice with peritoneal macrophages from syngeneic uninfected mice. The RBC from RLV-infected mice were recognized and phagocytized by these macrophages, as were 'old', but not 'young', RBC from uninfected mice. Neuraminidase treatment of RBC from RLV-infected mice had little effect on the extent of phagocytosis. Trypsin treatment generally, although not inevitably, reduced the phagocytosis of such RBC. The level of phagocytosis of RBC by mineral oil- or thioglycollate stimulated peritoneal macrophages was considerably lower than that of unstimulated macrophages (untreated>mineral oil>thioglycollate). Trypsin treatment of macrophages almost completely eliminated their capacity to recognize and phagocytize RBC from RLV infected mice. It appears that macrophages from normal, uninfected mice are capable of recognizing and phagocytizing RBC from RLV-infected mice as well as normal, 'old' RBC. As the authors have shown for normal 'old' RBC, the recognition of RBC from RLV-infected mice seems to be mediated via a trypsinsensitive receptor on the surface of the macrophages. The in vitro results strengthen the suggestion that macrophages in vivo play an important role in host defense against the development of Rauscher leukemia.
|Original language||English (US)|
|Number of pages||9|
|Journal||RES Journal of the Reticuloendothelial Society|
|State||Published - Dec 1 1978|
All Science Journal Classification (ASJC) codes