Phosphoinositide breakdown in isolated myelin is stimulated by GTP analogues and calcium

F. Golly; J. N. Larocca; R. W. Ledeen

doi:10.1002/jnr.490270313

Phosphoinositide breakdown in isolated myelin is stimulated by GTP analogues and calcium

F. Golly, J. N. Larocca, R. W. Ledeen

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Purified myelin from rat brainstem, prelabeled in vivo by intracerebral injection of [³H]myoinositol, showed enhanced breakdown of phosphoinositides on treatment with 5'‐guanylylimidodiphosphate [Gpp‐(NH)p] and Ca²⁺. Concentration variation of the former in the presence of Ca²⁺ snowed a dose‐dependent release of inositol 1,4‐bisphosphate (IP₂) and inositol 1,4,5‐trisphosphate (IP₃), while inositol 1‐phosphate (IP) release was erratic. Concentration‐dependent release of IP₂ and IP₃ was also observed with Ca²⁺ as the variable in the presence of Gpp(NH)p. Carbachol, when present, did not enhance the stimulatory effect of Gpp(NH)p alone. Addition of diphosphoglycerate during incubation enhanced IP₃ at the expense of IP₂, suggesting the presence of IP₃ phosphatase in mylein.

Original language	English (US)
Pages (from-to)	342-348
Number of pages	7
Journal	Journal of Neuroscience Research
Volume	27
Issue number	3
DOIs	https://doi.org/10.1002/jnr.490270313
State	Published - Nov 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Cellular and Molecular Neuroscience

Keywords

inositol phosphates
muscarinic receptors
myelin
phosphoinositides

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10.1002/jnr.490270313

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title = "Phosphoinositide breakdown in isolated myelin is stimulated by GTP analogues and calcium",

abstract = "Purified myelin from rat brainstem, prelabeled in vivo by intracerebral injection of [3H]myoinositol, showed enhanced breakdown of phosphoinositides on treatment with 5'‐guanylylimidodiphosphate [Gpp‐(NH)p] and Ca2+. Concentration variation of the former in the presence of Ca2+ snowed a dose‐dependent release of inositol 1,4‐bisphosphate (IP2) and inositol 1,4,5‐trisphosphate (IP3), while inositol 1‐phosphate (IP) release was erratic. Concentration‐dependent release of IP2 and IP3 was also observed with Ca2+ as the variable in the presence of Gpp(NH)p. Carbachol, when present, did not enhance the stimulatory effect of Gpp(NH)p alone. Addition of diphosphoglycerate during incubation enhanced IP3 at the expense of IP2, suggesting the presence of IP3 phosphatase in mylein.",

keywords = "inositol phosphates, muscarinic receptors, myelin, phosphoinositides",

author = "F. Golly and Larocca, {J. N.} and Ledeen, {R. W.}",

year = "1990",

month = nov,

doi = "10.1002/jnr.490270313",

language = "English (US)",

volume = "27",

pages = "342--348",

journal = "Journal of Neuroscience Research",

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AU - Golly, F.

AU - Larocca, J. N.

AU - Ledeen, R. W.

PY - 1990/11

Y1 - 1990/11

N2 - Purified myelin from rat brainstem, prelabeled in vivo by intracerebral injection of [3H]myoinositol, showed enhanced breakdown of phosphoinositides on treatment with 5'‐guanylylimidodiphosphate [Gpp‐(NH)p] and Ca2+. Concentration variation of the former in the presence of Ca2+ snowed a dose‐dependent release of inositol 1,4‐bisphosphate (IP2) and inositol 1,4,5‐trisphosphate (IP3), while inositol 1‐phosphate (IP) release was erratic. Concentration‐dependent release of IP2 and IP3 was also observed with Ca2+ as the variable in the presence of Gpp(NH)p. Carbachol, when present, did not enhance the stimulatory effect of Gpp(NH)p alone. Addition of diphosphoglycerate during incubation enhanced IP3 at the expense of IP2, suggesting the presence of IP3 phosphatase in mylein.

AB - Purified myelin from rat brainstem, prelabeled in vivo by intracerebral injection of [3H]myoinositol, showed enhanced breakdown of phosphoinositides on treatment with 5'‐guanylylimidodiphosphate [Gpp‐(NH)p] and Ca2+. Concentration variation of the former in the presence of Ca2+ snowed a dose‐dependent release of inositol 1,4‐bisphosphate (IP2) and inositol 1,4,5‐trisphosphate (IP3), while inositol 1‐phosphate (IP) release was erratic. Concentration‐dependent release of IP2 and IP3 was also observed with Ca2+ as the variable in the presence of Gpp(NH)p. Carbachol, when present, did not enhance the stimulatory effect of Gpp(NH)p alone. Addition of diphosphoglycerate during incubation enhanced IP3 at the expense of IP2, suggesting the presence of IP3 phosphatase in mylein.

KW - inositol phosphates

KW - muscarinic receptors

KW - myelin

KW - phosphoinositides

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ER -

Phosphoinositide breakdown in isolated myelin is stimulated by GTP analogues and calcium

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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