Phosphorylation of microtubule-associated proteins by protein kinase CK2 in neuritogenesis

Jesús Avila, Luis Ulloa, Josefa González, Francisco Moreno, Javier Diaz-Nido

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Phosphorylation of microtubule-associated protein MAP1B and the neuronal-specific βIII-tubulin isoform takes place during neurite growth in neuroblastoma cells. Protein kinase CK2 (formerly referred to as casein kinase 2) is possibly involved in βIII-tubulin phosphorylation. As for MAP1B, there are at least two types of phosphorylation; one catalyzed by proline-directed protein kinases and another catalyzed by CK2. Protein kinase CK2 is primarily localized to the nuclei in proliferating neuroblastoma cells, whereas an increased amount of the enzyme is present in the cytoplasm of postmitotic cells bearing neurites. Treatment of neuroblastoma cells with an antisense oligonucleotide which specifically results in CK2 catalytic subunit depletion inhibits neuritogenesis. CK2 depletion is accompanied by dephosphorylation of MAP1B on the corresponding phosphorylatable sites. This dephosphorylation is paralleled by a release of MAP1B from microtubules. These results suggest that MAP1B phosphorylation by CK2 may be required for the assembly of microtubules within neurites. Other neuronal cytoskeletal proteins including MAP1A and tau are also substrates for CK2, indicating a role for the enzyme in the regulation of cytoskeletal functions also in mature neurons.

Original languageEnglish (US)
Pages (from-to)573-579
Number of pages7
JournalCellular and Molecular Biology Research
Volume40
Issue number5-6
StatePublished - Dec 1 1994

Fingerprint

Casein Kinase II
Phosphorylation
Microtubule-Associated Proteins
Neurites
Neuroblastoma
Tubulin
Microtubules
Proline-Directed Protein Kinases
Bearings (structural)
Cytoskeletal Proteins
Antisense Oligonucleotides
Enzymes
Neurons
Catalytic Domain
Protein Isoforms
Cytoplasm
microtubule-associated protein 1B
Substrates
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Keywords

  • Casein kinase 2
  • Cytoskeleton
  • MAPIB
  • Microtubule-associated proteins
  • Neurite growth
  • Neuroblastoma cells
  • Protein phosphorylation
  • Tubulin

Cite this

Avila, Jesús ; Ulloa, Luis ; González, Josefa ; Moreno, Francisco ; Diaz-Nido, Javier. / Phosphorylation of microtubule-associated proteins by protein kinase CK2 in neuritogenesis. In: Cellular and Molecular Biology Research. 1994 ; Vol. 40, No. 5-6. pp. 573-579.
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Phosphorylation of microtubule-associated proteins by protein kinase CK2 in neuritogenesis. / Avila, Jesús; Ulloa, Luis; González, Josefa; Moreno, Francisco; Diaz-Nido, Javier.

In: Cellular and Molecular Biology Research, Vol. 40, No. 5-6, 01.12.1994, p. 573-579.

Research output: Contribution to journalArticle

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