Phosphorylation of smads by intracellular kinases

Fang Liu, Isao Matsuura

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Smad proteins transduce the TGF-ß family signal at the cell surface into gene regulation in the nucleus. In addition to being phosphorylated by the TGF-ß family receptors, Smads are phosphorylated by a variety of intracellular kinases. The most studied are by cyclin-dependent kinases, the MAP kinase family members, and GSK-3. Phosphorylation by these kinases regulates Smad activities, leading to various biological effects. This chapter describes the methods for analyzing Smad phosphorylation by these kinases.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
Publication statusPublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


  • CDKs
  • GSK-3
  • MAPKs
  • Phosphorylation
  • Smad

Cite this

Liu, F., & Matsuura, I. (2016). Phosphorylation of smads by intracellular kinases. In Methods in Molecular Biology (pp. 93-109). (Methods in Molecular Biology; Vol. 1344). Humana Press Inc..