Phosphorylation of the elongation factor 2: The fifth Ca2+ / calmodulin-dependent system of protein phosphorylation

Alexey Ryazanov, P. G. Natapov, E. A. Shestakova, F. F. Severin, A. S. Spirin

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Elongation factor 2 (EF-2) has been recently shown to be extensively phosphorylated in a Ca2+ / calmodulin-dependent manner in extracts of mammalian cells (A. G. Ryazanov (1987) FEBS Lett. 214, 331-334). In the present study, we partially purified the protein kinase which phosphorylates EF-2 from rabbit reticulocytes. The molecular weight of the enzyme determined by gel filtration was about 140 000. Unlike the substrate, the EF-2 kinase had no affinity for RNA and therefore could be separated from EF-2 by chromatography on RNA-Sepharose. After chromatography on hydroxyapatite, the kinase activity became calmodulin-dependent. Two-dimensional separation of the phosphorylated EF-2 according to O'Farrell's technique revealed that there were two phosphorylation sites within the EF-2 molecule; in both cases, the phosphorylated amino acid was threonine. The EF-2 kinase differed from the four known types of Ca2+ / calmodulin-dependent protein kinases. Thus, the system of EF-2 phosphorylation represents the novel (fifth) Ca2+ / calmodulin-dependent system of protein phosphorylation. This system is supposed to be responsible for the regulation of the elongation rate of protein biosynthesis in eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)619-626
Number of pages8
JournalBiochimie
Volume70
Issue number5
DOIs
StatePublished - Jan 1 1988

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Peptide Elongation Factor 2
Phosphorylation
Calmodulin
Elongation Factor 2 Kinase
Proteins
Chromatography
Translational Peptide Chain Elongation
RNA
Gastrin-Secreting Cells
Calcium-Calmodulin-Dependent Protein Kinases
Reticulocytes
Biosynthesis
Eukaryotic Cells
Threonine
Durapatite
Cell Extracts
Sepharose
Protein Kinases
Gel Chromatography
Elongation

All Science Journal Classification (ASJC) codes

  • Biochemistry

Keywords

  • calmodulin-dependent protein kinase
  • elongation factor 2/ Ca
  • protein phosphorylation
  • regulation of translation

Cite this

Ryazanov, Alexey ; Natapov, P. G. ; Shestakova, E. A. ; Severin, F. F. ; Spirin, A. S. / Phosphorylation of the elongation factor 2 : The fifth Ca2+ / calmodulin-dependent system of protein phosphorylation. In: Biochimie. 1988 ; Vol. 70, No. 5. pp. 619-626.
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Phosphorylation of the elongation factor 2 : The fifth Ca2+ / calmodulin-dependent system of protein phosphorylation. / Ryazanov, Alexey; Natapov, P. G.; Shestakova, E. A.; Severin, F. F.; Spirin, A. S.

In: Biochimie, Vol. 70, No. 5, 01.01.1988, p. 619-626.

Research output: Contribution to journalArticle

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