Physiological response of Desulfurispirillum indicum S5 to arsenate and nitrate as terminal electron acceptors

Ines Rauschenbach, Elisabetta Bini, Max M. Häggblom, Nathan Yee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The ability of anaerobic prokaryotes to employ different terminal electron acceptors for respiration enables these organisms to flourish in subsurface ecosystems. Desulfurispirillum indicum strain S5 is an obligate anaerobic bacterium that is able to grow by respiring a range of different electron acceptors, including arsenate and nitrate. Here, we examined the growth, electron acceptor utilization, and gene expression of D. indicum growing under arsenate and nitrate-reducing conditions. Consistent with thermodynamic predictions, the experimental results showed that the reduction of nitrate to ammonium yielded higher cell densities than the reduction of arsenate to arsenite. However, D. indicum grew considerably faster by respiration on arsenate compared with nitrate, with doubling times of 4.3 ± 0.2 h and 19.2 ± 2.0 h, respectively. Desulfurispirillum indicum growing on both electron acceptors exhibited the preferential utilization of arsenate before nitrate. The expression of the arsenate reductase gene arrA was up-regulated approximately 100-fold during arsenate reduction, as determined by qRT-PCR. Conversely, the nitrate reductase genes narG and napA were not differentially regulated under the conditions tested. The results of this study suggest that physiology, rather than thermodynamics, controls the growth rates and hierarchy of electron acceptor utilization in D. indicum.

Original languageEnglish (US)
Pages (from-to)156-162
Number of pages7
JournalFEMS microbiology ecology
Volume81
Issue number1
DOIs
StatePublished - Jul 1 2012

Fingerprint

arsenate
physiological response
Nitrates
Electrons
nitrate
electron
Thermodynamics
Arsenate Reductases
Respiration
respiration
thermodynamics
Nitrate Reductase
Anaerobic Bacteria
Growth
arsenite
gene
prokaryote
Genes
Ecosystem
arsenic acid

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Keywords

  • Arsenic
  • Electron tower
  • Geomicrobiology
  • Molybdoenzymes
  • Selenate

Cite this

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title = "Physiological response of Desulfurispirillum indicum S5 to arsenate and nitrate as terminal electron acceptors",
abstract = "The ability of anaerobic prokaryotes to employ different terminal electron acceptors for respiration enables these organisms to flourish in subsurface ecosystems. Desulfurispirillum indicum strain S5 is an obligate anaerobic bacterium that is able to grow by respiring a range of different electron acceptors, including arsenate and nitrate. Here, we examined the growth, electron acceptor utilization, and gene expression of D. indicum growing under arsenate and nitrate-reducing conditions. Consistent with thermodynamic predictions, the experimental results showed that the reduction of nitrate to ammonium yielded higher cell densities than the reduction of arsenate to arsenite. However, D. indicum grew considerably faster by respiration on arsenate compared with nitrate, with doubling times of 4.3 ± 0.2 h and 19.2 ± 2.0 h, respectively. Desulfurispirillum indicum growing on both electron acceptors exhibited the preferential utilization of arsenate before nitrate. The expression of the arsenate reductase gene arrA was up-regulated approximately 100-fold during arsenate reduction, as determined by qRT-PCR. Conversely, the nitrate reductase genes narG and napA were not differentially regulated under the conditions tested. The results of this study suggest that physiology, rather than thermodynamics, controls the growth rates and hierarchy of electron acceptor utilization in D. indicum.",
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Physiological response of Desulfurispirillum indicum S5 to arsenate and nitrate as terminal electron acceptors. / Rauschenbach, Ines; Bini, Elisabetta; Häggblom, Max M.; Yee, Nathan.

In: FEMS microbiology ecology, Vol. 81, No. 1, 01.07.2012, p. 156-162.

Research output: Contribution to journalArticle

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