TY - JOUR
T1 - Plasposons
T2 - Modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes
AU - Dennis, Jonathan J.
AU - Zylstra, Gerben J.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1998/7
Y1 - 1998/7
N2 - A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon's site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiotic resistance cassettes. The broad host range and low target DNA sequence specificity of the Tn5 transposase, in combination with the flexibility afforded by the modular arrangement of TnMod, result in a versatile tool for the mapping of insertional mutations and the rapid recovery of clones from gram-negative bacteria.
AB - A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon's site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiotic resistance cassettes. The broad host range and low target DNA sequence specificity of the Tn5 transposase, in combination with the flexibility afforded by the modular arrangement of TnMod, result in a versatile tool for the mapping of insertional mutations and the rapid recovery of clones from gram-negative bacteria.
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U2 - 10.1128/aem.64.7.2710-2715.1998
DO - 10.1128/aem.64.7.2710-2715.1998
M3 - Article
C2 - 9647854
AN - SCOPUS:0031869639
VL - 64
SP - 2710
EP - 2715
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 7
ER -