TY - JOUR
T1 - Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea
AU - Skarjinskaia, Marina
AU - Svab, Zora
AU - Maliga, Pal
N1 - Funding Information:
We thank Priya Sriraman for taking the confocal images and Arun Azhagiri for advice on processing the color images in Figure 2. This research was supported by funds from Monsanto Co. and Rutgers Special Project Grant No. 2-888198.
PY - 2003/2
Y1 - 2003/2
N2 - A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA 16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformation of 51 Lesquerella leaf samples, followed by spectinomycin selection, yielded two transplastomic clones. The AAD-GFP fusion protein, the marker gene product, was localized to chloroplasts by confocal laser microscopy. Fertile plants and seed progeny were obtained in line Lf-pZS391B-1. In the 51 samples a large number (108) of spontaneous mutants were identified. In five of the lines spectinomycin resistance was localized to a conserved stem structure by sequencing 16S rRNA genes. Success in L. fendleri, a wild oilseed species, extends plastid transformation beyond Arabidopsis thaliana in the Brassicaceae family.
AB - A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA 16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformation of 51 Lesquerella leaf samples, followed by spectinomycin selection, yielded two transplastomic clones. The AAD-GFP fusion protein, the marker gene product, was localized to chloroplasts by confocal laser microscopy. Fertile plants and seed progeny were obtained in line Lf-pZS391B-1. In the 51 samples a large number (108) of spontaneous mutants were identified. In five of the lines spectinomycin resistance was localized to a conserved stem structure by sequencing 16S rRNA genes. Success in L. fendleri, a wild oilseed species, extends plastid transformation beyond Arabidopsis thaliana in the Brassicaceae family.
KW - Lesquerella fendleri
KW - Plastid transformation
KW - Spectinomycin resistance
KW - Spectinomycin selection
KW - rRNA mutations
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U2 - 10.1023/A:1022110402302
DO - 10.1023/A:1022110402302
M3 - Article
C2 - 12650530
AN - SCOPUS:0038736839
SN - 0962-8819
VL - 12
SP - 115
EP - 122
JO - Transgenic Research
JF - Transgenic Research
IS - 1
ER -