TY - GEN
T1 - Poloxamer-188 improves capillary blood flow in the zone of stasis after burn injury
AU - Baskaran, H.
AU - Yarmush, M.
AU - Berthiaume, F.
PY - 1999
Y1 - 1999
N2 - Cutaneous burn injury causes blood flow reduction in regions near the site of the injury, collectively termed as the zone of stasis. Blood flow, in this zone, ceases after 24-48 hours resulting in an expansion of the injured area. We used intravital microscopy to investigate the zone of stasis. CD-1 male mice (30 g) were fitted with skin flap chambers and capillary blood flow was visualized by intravenously injecting fluorescently labeled red blood cells into the mice. A temperature-controlled copper tip of 2 mm diameter was used to inflict a full-thickness burn injury on the mouse skin. Real time fluorescent images of the blood flow before and after the burn were recorded. Playback image analysis showed that there was a reduction in blood flow near the site of burn injury immediately (0-2 hrs.) after the injury and away from the site of injury there was no change. Intravenous injection of Poloxamer-188 (0.1 ml, 200 mg/kg) greatly improved blood flow in the zone of stasis.
AB - Cutaneous burn injury causes blood flow reduction in regions near the site of the injury, collectively termed as the zone of stasis. Blood flow, in this zone, ceases after 24-48 hours resulting in an expansion of the injured area. We used intravital microscopy to investigate the zone of stasis. CD-1 male mice (30 g) were fitted with skin flap chambers and capillary blood flow was visualized by intravenously injecting fluorescently labeled red blood cells into the mice. A temperature-controlled copper tip of 2 mm diameter was used to inflict a full-thickness burn injury on the mouse skin. Real time fluorescent images of the blood flow before and after the burn were recorded. Playback image analysis showed that there was a reduction in blood flow near the site of burn injury immediately (0-2 hrs.) after the injury and away from the site of injury there was no change. Intravenous injection of Poloxamer-188 (0.1 ml, 200 mg/kg) greatly improved blood flow in the zone of stasis.
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M3 - Conference contribution
AN - SCOPUS:0033341446
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 775
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -