Population structure and phylogenetic characterization of marine benthic Archaea in deep-sea sediments

Costantino Vetriani, Holger W. Jannasch, Barbara J. Macgregor, David A. Stahl, Anna Louise Reysenbach

Research output: Contribution to journalArticle

345 Citations (Scopus)

Abstract

During the past few years Archaea have been recognized as a widespread and significant component of marine picoplankton assemblages and, more recently, the presence of novel archaeal phylogenetic lineages has been reported in coastal marine benthic environments. We investigated the relative abundance, vertical distribution, phylogenetic composition, and spatial variability of Archaea in deep-sea sediments collected from several stations in the Atlantic Ocean. Quantitative oligonucleotide hybridization experiments indicated that the relative abundance of archaeal 16S rRNA in deep-sea sediments (1500 m deep) ranged from about 2.5 to 8% of the total prokaryotic rRNA. Clone libraries of PCR- amplified archaeal rRNA genes (rDNA) were constructed from 10 depth intervals obtained from sediment cores collected at depths of 1,500, 2,600, and 4,500 m. Phylogenetic analysis of rDNA sequences revealed the presence of a complex archaeal population structure, whose members could be grouped into discrete phylogenetic lineages within the two kingdoms, Crenarchaeota and Euryarchaeota. Comparative denaturing gradient gel electrophoresis profile analysis of archaeal 16S rDNA V3 fragments revealed a significant depth-related variability in the composition of the archaeal population.

Original languageEnglish (US)
Pages (from-to)4375-4384
Number of pages10
JournalApplied and environmental microbiology
Volume65
Issue number10
StatePublished - Oct 1 1999

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deep-sea sediment
Archaea
Ribosomal DNA
Oceans and Seas
population structure
phylogenetics
sediments
Archaeal Genes
phylogeny
ribosomal RNA
Crenarchaeota
Euryarchaeota
Atlantic Ocean
Population
relative abundance
Denaturing Gradient Gel Electrophoresis
benthic environment
rRNA Genes
benthic zone
Oligonucleotides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Vetriani, C., Jannasch, H. W., Macgregor, B. J., Stahl, D. A., & Reysenbach, A. L. (1999). Population structure and phylogenetic characterization of marine benthic Archaea in deep-sea sediments. Applied and environmental microbiology, 65(10), 4375-4384.
Vetriani, Costantino ; Jannasch, Holger W. ; Macgregor, Barbara J. ; Stahl, David A. ; Reysenbach, Anna Louise. / Population structure and phylogenetic characterization of marine benthic Archaea in deep-sea sediments. In: Applied and environmental microbiology. 1999 ; Vol. 65, No. 10. pp. 4375-4384.
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Vetriani, C, Jannasch, HW, Macgregor, BJ, Stahl, DA & Reysenbach, AL 1999, 'Population structure and phylogenetic characterization of marine benthic Archaea in deep-sea sediments', Applied and environmental microbiology, vol. 65, no. 10, pp. 4375-4384.

Population structure and phylogenetic characterization of marine benthic Archaea in deep-sea sediments. / Vetriani, Costantino; Jannasch, Holger W.; Macgregor, Barbara J.; Stahl, David A.; Reysenbach, Anna Louise.

In: Applied and environmental microbiology, Vol. 65, No. 10, 01.10.1999, p. 4375-4384.

Research output: Contribution to journalArticle

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