Portable, battery-operated, low-cost, bright field and fluorescence microscope

Andrew R. Miller, Gregory L. Davis, Z. Maria Oden, Mohamad Reza Razavi, Abolfazl Fateh, Morteza Ghazanfari, Farid Abdolrahimi, Shahin Poorazar, Fatemeh Sakhaie, Randall J. Olsen, Ahmad Reza Bahrmand, Mark C. Pierce, Edward A. Graviss, Rebecca Richards-Kortum

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Abstract

This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 μm at 1000 × magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by the human pathogen, Mycobacterium tuberculosis. Sixty-four direct, decontaminated, and serially diluted smears were prepared from sputa obtained from 19 patients suspected to have M. tuberculosis infection. Slides were stained with auramine orange and evaluated as being positive or negative for M. tuberculosis with both the new portable fluorescence microscope and a laboratory grade fluorescence microscope. Concordant results were obtained in 98.4% of cases. This highly portable, low cost, fluorescence microscope may be a useful diagnostic tool to expand the availability of M. tuberculosis testing at the point-of-care in low resource settings.

Original languageEnglish (US)
Article numbere11890
JournalPloS one
Volume5
Issue number8
DOIs
StatePublished - Oct 15 2010
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Miller, A. R., Davis, G. L., Oden, Z. M., Razavi, M. R., Fateh, A., Ghazanfari, M., Abdolrahimi, F., Poorazar, S., Sakhaie, F., Olsen, R. J., Bahrmand, A. R., Pierce, M. C., Graviss, E. A., & Richards-Kortum, R. (2010). Portable, battery-operated, low-cost, bright field and fluorescence microscope. PloS one, 5(8), [e11890]. https://doi.org/10.1371/journal.pone.0011890