Post-transcriptional control of tryptophan hydroxylase gene expression in rat brain stem and pineal gland

Ronald P. Hart, Renji Yang, Leigh A. Riley, Todd L. Green

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have cloned a segment of the rat tryptophan hydroxylase (TPH) gene and used it to investigate differences in mRNA levels in two tissues: brain stem and pineal gland. The cloned, 13-kb genomic region contains five exons corresponding to cloned TPH cDNA sequence. Most intron/axon junctions are homologous to those found in the closely related genes encoding tyrosine and phenylalanine hydroxylase. The gene segment contains a region of low complexity that is repeated in the genome, but the remainder of the segment is single-copy sequence. TPH mRNAs can be detected by RNase protection assays and demonstrate a large difference in steady-state mRNA levels between pineal gland and brain stem dissections, agreeing with the results of Dumas and collaborators (1989, J. Neurosci. Res. 24:537-547). However, nuclear run-on assays of TPH gene transcription rates reveal similar levels of gene expression for pineal and brain stem. Control of TPH mRNA levels, therefore, appears to be post-transcriptional.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume2
Issue number1
DOIs
StatePublished - Feb 1991

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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