PR-Set7-dependent methylation of histone H4 Lys 20 functions in repression of gene expression and is essential for mitosis

Dmitry Karachentsev; Kavitha Sarma; Danny Reinberg; Ruth Steward

doi:10.1101/gad.1263005

PR-Set7-dependent methylation of histone H4 Lys 20 functions in repression of gene expression and is essential for mitosis

Dmitry Karachentsev, Kavitha Sarma, Danny Reinberg, Ruth Steward

Research output: Contribution to journal › Article › peer-review

155 Scopus citations

Abstract

The histone methyl transferase PR-Set7 mediates histone H4 Lys 20 methylation, a mark of constitutive and facultative heterochromatin. We isolated a null mutation in Drosophila PR-Set7 that suppresses position effect variegation, indicating that PR-Set7 indeed functions in silencing general gene expression. In PR-Set7 larval leg and eye discs, the number of cells is lower than normal, and the DNA content in these cells is significantly increased. These data show that PR-Set7-dependent methylation is essential for the process of mitosis, The methylation mark is highly stable and is maintained even in the absence of PR-Set7 protein.

Original language	English (US)
Pages (from-to)	431-435
Number of pages	5
Journal	Genes and Development
Volume	19
Issue number	4
DOIs	https://doi.org/10.1101/gad.1263005
State	Published - Feb 15 2005

All Science Journal Classification (ASJC) codes

Genetics
Developmental Biology

Keywords

Cell cycle and mitosis
Drosophila
Facultative and constitutive heterochromatin
Histone methylation
Transcriptional repression

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10.1101/gad.1263005

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AU - Sarma, Kavitha

AU - Reinberg, Danny

AU - Steward, Ruth

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AB - The histone methyl transferase PR-Set7 mediates histone H4 Lys 20 methylation, a mark of constitutive and facultative heterochromatin. We isolated a null mutation in Drosophila PR-Set7 that suppresses position effect variegation, indicating that PR-Set7 indeed functions in silencing general gene expression. In PR-Set7 larval leg and eye discs, the number of cells is lower than normal, and the DNA content in these cells is significantly increased. These data show that PR-Set7-dependent methylation is essential for the process of mitosis, The methylation mark is highly stable and is maintained even in the absence of PR-Set7 protein.

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KW - Facultative and constitutive heterochromatin

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KW - Transcriptional repression

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PR-Set7-dependent methylation of histone H4 Lys 20 functions in repression of gene expression and is essential for mitosis

Abstract

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