Pre-Steady-State Kinetic Analysis of Processive DNA Replication Including Complete Characterization of an Exonuclease-Deficient Mutant

Keyphrases

• DNA Replication 100%
• Processive 100%
• Conformational Change 100%
• Nucleotide Synthesis 100%
• Kinetic Analysis 100%
• Steady-state Kinetics 100%
• Exonuclease 100%
• Rate-limiting Step 66%
• DNA Primers 66%
• Excision 66%
• Single-stranded DNA (ssDNA) 66%
• Exonuclease Activity 66%
• Single-turnover Kinetics 66%
• Equilibrium Constant 66%
• Phosphorolysis 66%
• T7 DNA Polymerase 66%
• Single nucleotide 66%
• Pulse-chase Experiment 33%
• DNA Polymerase 33%
• Direct Measurement 33%
• Data-centric 33%
• Reverse Reaction 33%
• Polymerization Reaction 33%
• Ternary Complex 33%
• DNA Polymerization 33%
• Transition State 33%
• Fast Rates 33%
• Tight-binding 33%
• Pyrophosphate 33%
• Elemental Effects 33%
• Phosphodiester Bond Formation 33%
• Deoxyribonucleoside Triphosphates 33%
• Thermodynamic Description 33%
• Observed Rate Constant 33%
• Equilibrium Dissociation Constant 33%
• Double mutation 33%
• Individual Reactions 33%
• Transient State Analysis 33%
• Inorganic Pyrophosphate 33%
• Si Incorporation 33%

Biochemistry, Genetics and Molecular Biology

• Steady State 100%
• DNA Synthesis 100%
• Kinetics 100%
• Exonuclease 100%
• Conformational Change 75%
• Thymidine Triphosphate 75%
• Wild Type 50%
• Enzyme 50%
• DNA Polymerase 50%
• DNA Template 50%
• Pyrophosphate 50%
• T7 DNA Polymerase 50%
• Equilibrium Constant 50%
• Pulse Rate 25%
• Quantitative Technique 25%
• Ternary Complex 25%
• Single-Stranded DNA 25%
• DnaA 25%
• Phosphodiester Bond 25%
• Dissociation Constant 25%
• Observed Association Rate Constant 25%