Preparation of DNA and RNA fragments containing guanine N 2-thioalky1 tethers

Xiaorong Hou; Gang Wang; Barbara L. Gaffney; Roger A. Jones

doi:10.1002/0471142700.nc0508s41

Preparation of DNA and RNA fragments containing guanine N ²-thioalky1 tethers

Xiaorong Hou, Gang Wang, Barbara L. Gaffney, Roger A. Jones

SAS - Chem & Chemical Biology

Research output: Contribution to journal › Review article › peer-review

2 Scopus citations

Abstract

This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N² contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.

Original language	English (US)
Pages (from-to)	5.8.1-5.8.23
Journal	Current Protocols in Nucleic Acid Chemistry
Issue number	SUPPL. 41
DOIs	https://doi.org/10.1002/0471142700.nc0508s41
State	Published - Jun 2010

All Science Journal Classification (ASJC) codes

Biochemistry
Organic Chemistry

Keywords

disulfide cross-links
oligonucleotides

Access to Document

10.1002/0471142700.nc0508s41

Cite this

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title = "Preparation of DNA and RNA fragments containing guanine N 2-thioalky1 tethers",

abstract = "This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N2 contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.",

keywords = "disulfide cross-links, oligonucleotides",

author = "Xiaorong Hou and Gang Wang and Gaffney, {Barbara L.} and Jones, {Roger A.}",

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T1 - Preparation of DNA and RNA fragments containing guanine N 2-thioalky1 tethers

AU - Hou, Xiaorong

AU - Wang, Gang

AU - Gaffney, Barbara L.

AU - Jones, Roger A.

PY - 2010/6

Y1 - 2010/6

N2 - This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N2 contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.

AB - This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N2 contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.

KW - disulfide cross-links

KW - oligonucleotides

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DO - 10.1002/0471142700.nc0508s41

M3 - Review article

C2 - 20517990

AN - SCOPUS:77953848911

SP - 5.8.1-5.8.23

JO - Current Protocols in Nucleic Acid Chemistry

JF - Current Protocols in Nucleic Acid Chemistry

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