Preparation of DNA and RNA fragments containing guanine N 2-thioalky1 tethers

Xiaorong Hou, Gang Wang, Barbara L. Gaffney, Roger A. Jones

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations


This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N2 contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.

Original languageEnglish (US)
Pages (from-to)5.8.1-5.8.23
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL. 41
StatePublished - Jun 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry


  • disulfide cross-links
  • oligonucleotides


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