Preparation of neonatal rat Schwann cells and embryonic dorsal root ganglia neurons for in vitro myelination studies

Patrice Maurel

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The ability to understand in great details, at the molecular level, the process of myelination in the peripheral nervous system (PNS) is, in no minor part, due to the availability of an in vitro culture model of PNS myelination. This culture system is based on the ability to prepare large population of highly purified Schwann cells and dorsal root ganglia neurons that, once co-cultured, can be driven to form in vitro well-defined myelinated axon units. In this chapter, we present our detailed protocols to establish these cell cultures that are derived from modifications of procedures developed 35–40 years ago.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages17-37
Number of pages21
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Co-culture
  • DRG
  • Dorsal root ganglia
  • Myelination
  • Neuron
  • Rat
  • Rodent
  • Schwann cell

Cite this

Maurel, P. (2018). Preparation of neonatal rat Schwann cells and embryonic dorsal root ganglia neurons for in vitro myelination studies. In Methods in Molecular Biology (pp. 17-37). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_2