Prevention of meningo/encephalomyelitis due to Sarcocystis neurona infection in mice is mediated by CD8 cells

Sharon G. Witonsky; Robert M. Gogal; Robert B. Duncan; Heather Norton; Dan Ward; David S. Lindsay

doi:10.1016/j.ijpara.2004.10.021

Prevention of meningo/encephalomyelitis due to Sarcocystis neurona infection in mice is mediated by CD8 cells

Sharon G. Witonsky, Robert M. Gogal, Robert B. Duncan, Heather Norton, Dan Ward, David S. Lindsay

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Immunodeficient CD8 knockout mice were infected with Sarcocystis neurona merozoites, in order to determine the role of CD8 cells in protective immunity. Using a direct agglutination test, all infected mice seroconverted by selected time points. Infected mice developed splenomegaly and bilateral lymphadenopathy. Histological changes included marked follicular development in the spleen, endothelitis and moderate perivascular inflammation in the liver, and meningoencephalitis in the brain. Infected brains were positive for S. neurona by polymerase chain reaction. Corresponding to histopathological changes, there were decreased numbers of B-cells in the spleen. The mice did not have significant memory (CD44hi/CD4) or effector (CD45RBhi/CD4) populations present at the time of euthanasia. Flow cytometry confirmed the lack of CDS cells. Taken together, these data support previous studies suggesting a critical role for CDS cells in the prevention of menigoencephalitis in S. neurona-infected mice.

Original language	English (US)
Pages (from-to)	113-123
Number of pages	11
Journal	International Journal for Parasitology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.ijpara.2004.10.021
State	Published - Jan 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Parasitology
Infectious Diseases

Keywords

CD8
Central nervous system
Equine immunology
Equine neurology
Equine protozoal myeloencephalitis
Mouse model
Sarcocystis neurona

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10.1016/j.ijpara.2004.10.021

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title = "Prevention of meningo/encephalomyelitis due to Sarcocystis neurona infection in mice is mediated by CD8 cells",

abstract = "Immunodeficient CD8 knockout mice were infected with Sarcocystis neurona merozoites, in order to determine the role of CD8 cells in protective immunity. Using a direct agglutination test, all infected mice seroconverted by selected time points. Infected mice developed splenomegaly and bilateral lymphadenopathy. Histological changes included marked follicular development in the spleen, endothelitis and moderate perivascular inflammation in the liver, and meningoencephalitis in the brain. Infected brains were positive for S. neurona by polymerase chain reaction. Corresponding to histopathological changes, there were decreased numbers of B-cells in the spleen. The mice did not have significant memory (CD44hi/CD4) or effector (CD45RBhi/CD4) populations present at the time of euthanasia. Flow cytometry confirmed the lack of CDS cells. Taken together, these data support previous studies suggesting a critical role for CDS cells in the prevention of menigoencephalitis in S. neurona-infected mice.",

keywords = "CD8, Central nervous system, Equine immunology, Equine neurology, Equine protozoal myeloencephalitis, Mouse model, Sarcocystis neurona",

author = "Witonsky, {Sharon G.} and Gogal, {Robert M.} and Duncan, {Robert B.} and Heather Norton and Dan Ward and Lindsay, {David S.}",

note = "Funding Information: This project was funded through an Animal Health and Disease grant (CSREES) to SGW, RMG, DSL and RBD.",

year = "2005",

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doi = "10.1016/j.ijpara.2004.10.021",

language = "English (US)",

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AU - Witonsky, Sharon G.

AU - Gogal, Robert M.

AU - Duncan, Robert B.

AU - Norton, Heather

AU - Ward, Dan

AU - Lindsay, David S.

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N2 - Immunodeficient CD8 knockout mice were infected with Sarcocystis neurona merozoites, in order to determine the role of CD8 cells in protective immunity. Using a direct agglutination test, all infected mice seroconverted by selected time points. Infected mice developed splenomegaly and bilateral lymphadenopathy. Histological changes included marked follicular development in the spleen, endothelitis and moderate perivascular inflammation in the liver, and meningoencephalitis in the brain. Infected brains were positive for S. neurona by polymerase chain reaction. Corresponding to histopathological changes, there were decreased numbers of B-cells in the spleen. The mice did not have significant memory (CD44hi/CD4) or effector (CD45RBhi/CD4) populations present at the time of euthanasia. Flow cytometry confirmed the lack of CDS cells. Taken together, these data support previous studies suggesting a critical role for CDS cells in the prevention of menigoencephalitis in S. neurona-infected mice.

AB - Immunodeficient CD8 knockout mice were infected with Sarcocystis neurona merozoites, in order to determine the role of CD8 cells in protective immunity. Using a direct agglutination test, all infected mice seroconverted by selected time points. Infected mice developed splenomegaly and bilateral lymphadenopathy. Histological changes included marked follicular development in the spleen, endothelitis and moderate perivascular inflammation in the liver, and meningoencephalitis in the brain. Infected brains were positive for S. neurona by polymerase chain reaction. Corresponding to histopathological changes, there were decreased numbers of B-cells in the spleen. The mice did not have significant memory (CD44hi/CD4) or effector (CD45RBhi/CD4) populations present at the time of euthanasia. Flow cytometry confirmed the lack of CDS cells. Taken together, these data support previous studies suggesting a critical role for CDS cells in the prevention of menigoencephalitis in S. neurona-infected mice.

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KW - Equine protozoal myeloencephalitis

KW - Mouse model

KW - Sarcocystis neurona

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Prevention of meningo/encephalomyelitis due to Sarcocystis neurona infection in mice is mediated by CD8 cells

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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