Primary structure of the β-subunit of bovine transducin deduced from the cDNA sequence

Katsunori Sugimoto; Toshihide Nukada; Tsutomu Tanabe; Hideo Takahashi; Masaharu Noda; Naoto Minamino; Kenji Kangawa; Hisayuki Matsuo; Tadaaki Hirose; Seiichi Inayama; Shosaku Numa

doi:10.1016/0014-5793(85)80015-6

Primary structure of the β-subunit of bovine transducin deduced from the cDNA sequence

Katsunori Sugimoto, Toshihide Nukada, Tsutomu Tanabe, Hideo Takahashi, Masaharu Noda, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Tadaaki Hirose, Seiichi Inayama, Shosaku Numa

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

DNA complementary to the bovine retinal mRNA coding for the β-subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 340 amino acid residues (including the initiating methionine). Furthermore, cDNA hybridizable with a transducin β-subunit cDNA probe has been cloned from a library derived from bovine brain poly(A)⁺ RNA. Comparison of the cloned cDNAs, in conjunction with blot hybridization analysis and S₁ nuclease mapping of poly(A)⁺ RNA from bovine retina, brain and liver, suggests that the mRNAs coding for the β-subunits of transducin and other guanine nucleotide binding proteins have the same protein-coding sequence but partly different 5'-noncoding sequences.

Original language	English (US)
Pages (from-to)	235-240
Number of pages	6
Journal	FEBS Letters
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80015-6
State	Published - Oct 28 1985
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Keywords

Guanine nucleotide binding protein
Nucleotide sequence RNA blot hybridization analysis S nuclease mapping
Transducin
cDNA cloning

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10.1016/0014-5793(85)80015-6

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title = "Primary structure of the β-subunit of bovine transducin deduced from the cDNA sequence",

abstract = "DNA complementary to the bovine retinal mRNA coding for the β-subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 340 amino acid residues (including the initiating methionine). Furthermore, cDNA hybridizable with a transducin β-subunit cDNA probe has been cloned from a library derived from bovine brain poly(A)+ RNA. Comparison of the cloned cDNAs, in conjunction with blot hybridization analysis and S1 nuclease mapping of poly(A)+ RNA from bovine retina, brain and liver, suggests that the mRNAs coding for the β-subunits of transducin and other guanine nucleotide binding proteins have the same protein-coding sequence but partly different 5'-noncoding sequences.",

keywords = "Guanine nucleotide binding protein, Nucleotide sequence RNA blot hybridization analysis S nuclease mapping, Transducin, cDNA cloning",

author = "Katsunori Sugimoto and Toshihide Nukada and Tsutomu Tanabe and Hideo Takahashi and Masaharu Noda and Naoto Minamino and Kenji Kangawa and Hisayuki Matsuo and Tadaaki Hirose and Seiichi Inayama and Shosaku Numa",

note = "Funding Information: We thank Dr Paul Berg and Dr Hiroto Okayama for providingt heir high-efficiencyc loning system. This investigation was supported in part by researchg rants from the Ministry of Education, Science and Culture of Japan, the Mitsubishi Foundation and the Japanese Foundation of Metabolisma nd Diseases.",

year = "1985",

month = oct,

day = "28",

doi = "10.1016/0014-5793(85)80015-6",

language = "English (US)",

volume = "191",

pages = "235--240",

journal = "FEBS Letters",

issn = "0014-5793",

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number = "2",

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TY - JOUR

T1 - Primary structure of the β-subunit of bovine transducin deduced from the cDNA sequence

AU - Sugimoto, Katsunori

AU - Nukada, Toshihide

AU - Tanabe, Tsutomu

AU - Takahashi, Hideo

AU - Noda, Masaharu

AU - Minamino, Naoto

AU - Kangawa, Kenji

AU - Matsuo, Hisayuki

AU - Hirose, Tadaaki

AU - Inayama, Seiichi

AU - Numa, Shosaku

N1 - Funding Information: We thank Dr Paul Berg and Dr Hiroto Okayama for providingt heir high-efficiencyc loning system. This investigation was supported in part by researchg rants from the Ministry of Education, Science and Culture of Japan, the Mitsubishi Foundation and the Japanese Foundation of Metabolisma nd Diseases.

PY - 1985/10/28

Y1 - 1985/10/28

N2 - DNA complementary to the bovine retinal mRNA coding for the β-subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 340 amino acid residues (including the initiating methionine). Furthermore, cDNA hybridizable with a transducin β-subunit cDNA probe has been cloned from a library derived from bovine brain poly(A)+ RNA. Comparison of the cloned cDNAs, in conjunction with blot hybridization analysis and S1 nuclease mapping of poly(A)+ RNA from bovine retina, brain and liver, suggests that the mRNAs coding for the β-subunits of transducin and other guanine nucleotide binding proteins have the same protein-coding sequence but partly different 5'-noncoding sequences.

AB - DNA complementary to the bovine retinal mRNA coding for the β-subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 340 amino acid residues (including the initiating methionine). Furthermore, cDNA hybridizable with a transducin β-subunit cDNA probe has been cloned from a library derived from bovine brain poly(A)+ RNA. Comparison of the cloned cDNAs, in conjunction with blot hybridization analysis and S1 nuclease mapping of poly(A)+ RNA from bovine retina, brain and liver, suggests that the mRNAs coding for the β-subunits of transducin and other guanine nucleotide binding proteins have the same protein-coding sequence but partly different 5'-noncoding sequences.

KW - Guanine nucleotide binding protein

KW - Nucleotide sequence RNA blot hybridization analysis S nuclease mapping

KW - Transducin

KW - cDNA cloning

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Primary structure of the β-subunit of bovine transducin deduced from the cDNA sequence

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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