Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli

Haoran Zhang, Yong Wang, Brett Boghigian, Blaine A. Pfeifer

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Heterologous biosynthesis offers a new way to capture the medicinal properties presented by complex natural products. In this study, production of 6-deoxyerythronolide B (6dEB), the polyketide precursor to the antibiotic erythromycin, was used to probe the heterologous pathways needed for Escherichia coli-derived biosynthesis. More specifically, the heterologous proteins responsible for 6dEB production were varied by adjusting their respective gene dosage levels. In this way, heterologous components required for posttranslational modification, 6dEB biosynthesis, and substrate provision were adjusted in expression levels to observe the relative effect each has on final heterologous biosynthesis. The results indicate that both the biosynthetic and substrate provision heterologous proteins impact 6dEB formation to a greater extent when compared with posttranslational modification and suggest these components for future protein and metabolic engineering.

Original languageEnglish (US)
Pages (from-to)390-394
Number of pages5
JournalMicrobial Biotechnology
Volume2
Issue number3
DOIs
StatePublished - May 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli'. Together they form a unique fingerprint.

Cite this