Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens

Maria S. Mazanko, Maksim S. Makarenko, Vladimir A. Chistyakov, Alexander V. Usatov, Evgeniya V. Prazdnova, Anzhelika B. Bren, Ivan F. Gorlov, Zoya B. Komarova, Richard Weeks, Michael L. Chikindas

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.

Original languageEnglish (US)
Pages (from-to)1324-1329
Number of pages6
JournalProbiotics and Antimicrobial Proteins
Volume11
Issue number4
DOIs
StatePublished - Dec 1 2019

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine
  • Molecular Biology

Keywords

  • Bacillus
  • Poultry
  • Probiotic supplementation
  • Reproductive aging
  • Vitellogenin
  • vtg

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